Project description:To understand the molecular mechanism by which regulate skeletal development, we attempted to identify transcription factors that were highly expressed in developing cartilage during the embryonic stage. Col2a1-Venus-Tg mice in which chondrocytes were fluorescently labelled with the GFP-modified Venus gene. We purified total RNA from Venus-negative and Venus-positive cells and performed microarray analysis using an Affymetrix Mouse Genome 430 2.0 Array.
Project description:A human organoid culture system was set up to grow enteroendocrine cells with a venus labeled on the glucagon gene promoter sequence. This enabled the sorting of glucagon gene positive cells from negative cells, thereby enabling the enrichment of glucagon producing cells for study. Both Venus positive and venus negative cell populations were collected and their peptidome was assessed using nano LC-MS/MS
Project description:We purified two populations of human ileal enteroendocrine cells (Venus+ and Venus-) from mature differentiated hGLU-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages.
Project description:We purified two populations of human duodenal MLN-expressing enteroendocrine cells (Venus+ and Venus-) from mature differentiated hMLN-Venus organoids by FACS and identified transcripts enriched in endocrine cell lineages.
