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Rumen culture

ABSTRACT: Determining the culturability of the bovine rumen bacterial and archaeal microbiota

PROVIDER: PRJEB78542 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13580200_1.fastq.gz	Fastqsanger.gz
	ERR13580200_2.fastq.gz	Fastqsanger.gz
	ERR13580201_1.fastq.gz	Fastqsanger.gz
	ERR13580201_2.fastq.gz	Fastqsanger.gz
	ERR13580202_1.fastq.gz	Fastqsanger.gz

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Rumen metaproteomics - Catching the tip of the iceberg - evaluation of sample preparation protocols for metaproteomic studies of the rumen microbiota

Project description:Metaproteomic studies of the rumen microbiota are challenged by the need of optimized sample preparation protocols in order to retrieve an enhanced amount of prokaryotic instead of plant and bovine derived cells before protein extraction and subsequent LC-MS/MS analysis. The present study evaluates three different protocols applied to the rumen microbiota either attached to plant fibres or present as planktonic cells. The findings of our work suggest the integration of cheesecloth-gauze filtration in sample preparation to achieve a better protein identification ratio.

2015-04-01 | PXD001526 | Pride

The mechanism of promoting proliferation of rumen epithelial cells in Tan sheep by adding succinate to the diet based on the interaction between rumen microbiota and host.

Project description:A healthy rumen is crucial for normal growth and improved production performance of ruminant animals. Rumen microbes participate in and regulate rumen epithelial function, and the diverse metabolites produced by rumen microbes are important participants in rumen microbe-host interactions. SCFAs, as metabolites of rumen microbes, have been widely studied, and propionate and butyrate have been proven to promote rumen epithelial cell proliferation. Succinate, as an intermediate metabolite in the citric acid cycle, is a final product in the metabolism of certain rumen microbes, and is also an intermediate product in the microbial synthesis pathway of propionate. However, its effect on rumen microbes and rumen epithelial function has not been studied. It is unclear whether succinate can stimulate rumen epithelial development. Therefore, in this experiment, Chinese Tan sheep were used as experimental animals to conduct a comprehensive analysis of the rumen microbiota community structure and rumen epithelial transcriptome, to explore the role of adding succinate to the diet in the interaction between the rumen microbiota and host.

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Project description:The goal of this study was to characterize a single-cell clone derived from bovine rumen epithelium. Analyses including RNA-seq demonstrated that this clone was derived from a rumen epithelial cell. This clone is named BREC1 in the manuscript.

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Project description:SARST-V1 method was used to asses the effect of live yeast on the microbial population of the rumen of cows fed an acidogenic diet 3 cows were used in 3 by 3 latin-square design with 3 periods. In each period animals received either 0.5g/d of yeast, 5g/d of yeast or none. Rumen microbiota was analysed using the SARST-V1 method for each period.

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Rumen microbiome and effect of monensin

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Exposure of a bovine rumen microbiome model to glyphosate

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Essential oils affect populations of some rumen bacteria as revealed by microarray analysis

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Project description:bovine rumen metagenome Metagenome

| PRJNA454748 | ENA