Project description:Determination of differentially expressed genes in the proximal colon and distal ileum tissue in MR1 and IL-17A deficiency at steady-state. Tissue from naïve mice was harvested, total RNA extracted and subjected to RNASeq analysis.
Project description:Starting from the evidences of gut microbiota role in tumor development, progression and response to therapy, this project was aimed at evaluating the effect of antibiotic treatment on intestine. The intestinal flora of FVB mice was altered by the oral administration of vancomicyn, mice were then injected with MI6 cells, a singeneic tumor cells line established from spontanueous mammary tumor developped in transgenic d16HER2-transgenic mice [Castagnoli L, 2014]. To analyze the impact of vancomycin on intestinal mucosa, a gene expression profile was performed on colon and ileum samples.
Project description:Whole tissues corresponding to the ileum, colon and rectum were dissected from adult mice and used for RNA preparation. The aim of experiment was to study the impact of gut microbiota on gene expression in different gut regions. Experiment Overall Design: Three biological replicates were used for each condition. Each RNA preparation was independently hybridized to one chip.
Project description:Resequening ileum and colon epithelium with deeper reads to evaluate consequence of Satb2 gene loss in the colonic epithelium. The same library construction method was used to compare with Bulk RNA-seq data from In Vitro cultured mouse organoids.
