Project description:In order to obtain a global view on the transcriptional changes induced by the sex-inducing pheromone released by Seminavis robusta mating type plus cells (SIP+), an RNA-seq experiment was conducted. Therefore, purified SIP+ from RP-UPLC/MS was added to MT- cultures, 15 min before light onset after a prolonged dark period. MT- cultures without extract addition constituted the control conditions. Samples were taken after 15 min, 1 h and 3 h of illumination. An additional, non-treated MT- culture was harvested before illumination.

2016-03-22 | E-MTAB-4056 | biostudies-arrayexpress

Cylindrotheca closterium sexual reproduction transcriptome

Project description:A sexual reproduction transcriptome for the meroplanktonic diatom Cylindrotheca closterium

| PRJEB49955 | ENA

Transcriptome profiling of Cylindrotheca closterium and Seminavis robusta

Project description:Transcriptome profiling of Cylindrotheca closterium and Seminavis robusta

| PRJEB52586 | ENA

Cylindrotheca closterium, reference genome sequencing

Project description:Genomics data produced to generate a first reference draft of the Cylindrotheca closterium diatom species.

| PRJEB49951 | ENA

Seminavis_comparative

Project description:RNA-seq data representing the response of Seminavis robusta MT+ cells to a filtrate containing the sex pheromone SIP-.

| PRJEB35793 | ENA

Five diatoms transcriptomes under iron limitation and oxidative stress

Project description:Divergent functions of two clades of flavodoxin in diatoms mitigate oxidative stress and iron limitation Thalassiosira pseudonana and 4 open-ocean diatoms were subjected to iron limitation or short-term oxidative stress (hydrogen peroxide). mRNA profiles of T. pseudonana (CCMP1335), Thalassiosira oceanica (CCMP1005), Amphora coffeaeformis (CCMP1405), Chaetoceros sp. (CCMP199), and Cylindrotheca closterium (CCMP340).

2022-11-07 | GSE217467 | GEO

Pheromone Mediated Sexual Reproduction of Pennate Diatom Cylindrotheca closterium.

Project description:Benthic diatoms dominate primary production in marine subtidal and intertidal environments. Their extraordinary species diversity and ecological success is thought to be linked with their predominantly heterothallic sexual reproduction. Little is known about pheromone involvement during mating of pennate diatoms. Here we describe pheromone guided mating in the coastal raphid diatom Cylindrotheca closterium. We show that the two mating types (mt+ and mt-) have distinct functions. Similar to other benthic diatoms, mt+ cells are searching for the mt- cells to pair. To enhance mating efficiency mt- exudes an attraction pheromone which we proved by establishing a novel capillary assay. Further, two more pheromones produced by mt- promote the sexual events. One arrests the cell cycle progression of mt+ while the other induces gametogenesis of mt+. We suggest that C. closterium shares a functionally similar pheromone system with other pennate diatoms like Seminavis robusta and Pseudostaurosira trainorii which synchronize sexual events and mate attraction. Remarkably, we found no evidence of mt+ producing pheromones, which differentiates C. closterium from other pennates and suggests a less complex pheromone system in C. closterium.

| S-EPMC8217010 | biostudies-literature

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