Project description:To determine if there is differential gene expression response to Mycobacterium tuberculosis in neutrophils from persons living with HIV who remain tuberculin skin test (TST) and inteferon gamma release assay (IGRA) negative and have no tuberculosis history (HITTIN) compared to persons living with HIV and no previous TB but TST and IGRA positive (HIT).
Project description:To compare gene expression changes induced by infection with Mycobacterium tuberculosis (Mtb) with changes induced by purified Mtb products, we infected THP-1 cells with Mtb strain H37Rv or treated with purified Mtb products, then performed RNAseq.
Project description:Bulk RNAseq data looking at transcriptional differences between Mtb-specific T cells in the lung, vs those in the vasculature (with naive, CD44low controls) during Mycobacterium tuberculosis infection. Allowing us an unbiased way to discern differentially expressed genes in the two tissue spaces.
Project description:Trained Immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to specific microbial or metabolic components. In this study, monocytes were trained with beta-glucan and then re-exposed to Mycobacterium tuberculosis.
Project description:Comparison of Mycobacterium tuberculosis infection in WT mice strain C57BL/6 (WTM-BM-^]) and the CXCL5 knockout (KO) Two color arrays with dye swaps, RNA harvested at day 0 (d00, no infection) and day 21 (d21); 5 mice per experimental group. RNA from each experimental group was pooled. The e0, e8, e9 and eX are internal experimental run/labels of equivalent biological replications of the experiments.
Project description:Mycobacterium tuberculosis has specialised its metabolism to reside extra- and intra-cellularly in the human host. Nutrient availability and their concentrations can vary dramatically in these niches. Lactate is abundant during infection and it is an important signalling molecule regulating the immune response. In addition, lactate is abundant in blood, epithelial mucosa and a number of other relevant compartments in the host. Interestingly, previous studies have demonstrated that lactate and one of its possible first degradation products, pyruvate, allow superior growth compared to glucose and fatty acids in vitro for M. tuberculosis. Based on this information, we performed a "multi-omics" investigation to study the metabolism of pyruvate and lactate in M. tuberculosis. We employed TraDIS, transcriptomics, proteomics and stable isotopic labelling coupled with mass spectrometry-based metabolomics. Our studies reveal the structure of M. tuberculosis metabolic network associated with catabolism of pyruvate and lactate. Together these findings lead us to reconsider some well-accepted "truths" on carbon metabolism in M. tuberculosis.
