Project description:Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB), capable of manipulating and circumventing the host's immune system to establish infection. Ubiquitination plays a crucial role in the host's response to pathogens; however, the global alterations in protein ubiquitination during Mtb infection remain poorly understood. To elucidate the regulatory roles of ubiquitination in the immune response to Mtb, we investigated the ubiquitome of human macrophages following Mtb infection.
Project description:To determine if there is differential gene expression response to Mycobacterium tuberculosis in neutrophils from persons living with HIV who remain tuberculin skin test (TST) and inteferon gamma release assay (IGRA) negative and have no tuberculosis history (HITTIN) compared to persons living with HIV and no previous TB but TST and IGRA positive (HIT).
Project description:To compare gene expression changes induced by infection with Mycobacterium tuberculosis (Mtb) with changes induced by purified Mtb products, we infected THP-1 cells with Mtb strain H37Rv or treated with purified Mtb products, then performed RNAseq.
Project description:Trained Immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to specific microbial or metabolic components. In this study, monocytes were trained with beta-glucan and then re-exposed to Mycobacterium tuberculosis.
Project description:Bulk RNAseq data looking at transcriptional differences between Mtb-specific T cells in the lung, vs those in the vasculature (with naive, CD44low controls) during Mycobacterium tuberculosis infection. Allowing us an unbiased way to discern differentially expressed genes in the two tissue spaces.
Project description:Comparison of Mycobacterium tuberculosis infection in WT mice strain C57BL/6 (WTM-BM-^]) and the CXCL5 knockout (KO) Two color arrays with dye swaps, RNA harvested at day 0 (d00, no infection) and day 21 (d21); 5 mice per experimental group. RNA from each experimental group was pooled. The e0, e8, e9 and eX are internal experimental run/labels of equivalent biological replications of the experiments.
