Pseudorabies virus transcriptome sequencing
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR908011.fastq.gz | Fastqsanger.gz | |||
| ERR908011_1.fastq.gz | Fastqsanger.gz | |||
| ERR908011_2.fastq.gz | Fastqsanger.gz | |||
| ERR908012.fastq.gz | Fastqsanger.gz |
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Project description:<p>Pseudorabies virus infection causes intestinal flora disorder in animals, leading to diarrhea. To explore the effect of inactivated pseudorabies vaccine immunization on pseudorabies virus infection, this study sequenced the serum metabolome and intestinal microbiota of Hu sheep, analyzed their differential expression and conducted a combined analysis. The results showed that all the Hu sheep in the vaccine immunization group survived, while those in the non-immunization group died. The expressions of quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate, L-methionine, L-glutamic acid, thymine, adenine and 20-HETE were upregulated in the serum of the vaccine immunization group (P<0.05). The expressions of amifostine, hemialdehyde succinate and trehalose were down-regulated (P<0.05). The microbiota structure and abundance in the vaccine immunization group changed significantly, and the beneficial bacteria Christensenellaceae_R-7_group, Bifidobacterium, Solibacillus, and Rikenellaceae_RC9_gut_group increased. The results of the combined omics analysis showed that the changes of metabolites such as quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate and adenine were positively correlated with the abundance changes of Rikenellaceae_RC9_gut_group (P<0.05); L-methionine and 20-HETE were positively correlated with adenine and Solibacillus (P<0.05); Succinate semialdehyde was significantly negatively correlated with Rikenellaceae_RC9_gut_group. The above results indicate that after inactivated pseudorabies vaccine immunization of Hu sheep, it affects metabolites (quercetin, 3, 7-dimethylquercetin, 5-Methoxyindoleacetate adenine, L-methionine and 20-HETE) by maintaining beneficial intestinal bacteria (Rikenella aceae_RC9_group and Solibacillus). Metabolites jointly regulate the generation of inflammatory mediators, influence the function of immune cells and participate in the regulation of oxidative stress, thereby exerting an immune protective effect. This study provides an important theoretical basis for a deeper understanding of the immune protection mechanism of inactivated pseudorabies vaccine.</p>
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