Dose-dependent transcriptome expression of alcohol on oligodendrocytes
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ABSTRACT:
PROVIDER: PRJNA1000573 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR25467510.fastq.gz | Fastqsanger.gz | |||
| SRR25467511.fastq.gz | Fastqsanger.gz | |||
| SRR25467512.fastq.gz | Fastqsanger.gz | |||
| SRR25467513.fastq.gz | Fastqsanger.gz | |||
| SRR25467514.fastq.gz | Fastqsanger.gz |
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Project description:Oligodendrocytes (OLs) are glial cells within the central nervous system (CNS) that produce myelin, which coats neuronal axons and enhances fast propagation of neuronal signals. OLs are known to be severely affected by alcohol in fetal alcohol spectrum disorder (FASD) and alcohol use disorder (AUD). In FASD, oligodendrocyte precursor cells have been reported to be highly affected by any dose of alcohol. In AUD, high doses of alcohol can lead to demyelinating complications such as Wernicke-Korsakoff sybdrome. However, relatively little is known about how alcohol at low and high doses affects mature OLs (mOLs). To better understand the impact of concentration-dependent alcohol exposure on mOLs, we isolated and differentiated immature murine OLs into mOLs and exposed them to a moderate or high concentration of alcohol in vitro. RNA was then isolated from the cultures and sequenced using 3’-Tag RNA sequencing. Differential expression across alcohol concentration and control groups was calculated, which was used for gene set enrichment analysis to identify KEGG and GO terms that were significantly changed as a result of alcohol treatment. We found concentration dependent changes in biological pathways related to cell cycle regulation, translation initiation, integrin signaling, FGF2 signaling, inflammation, and myelination. Further, we found that the moderate and high concentrations of alcohol elicited distinct changes in gene expression, indicating that concentration is a critical factor when determining the effects of alcohol on transcriptomic changes.
2023-09-24 | GSE239691 | GEO
Project description:SHY2-dose dependent transcriptome analysis
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Project description:Age-dependent changes in translational expression of oligodendrocytes
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Project description:Rats were trained to orally self-administer alcohol in a concurrent, two-lever, free-choice contingency using a modification of the sweet solution fading procedure (O'Dell et al., 2004; Roberts et al., 2000; Vendruscolo et al., 2012). Following acquisition of self-administration, rats were allowed to self-administer unsweetened alcohol (10%) for 4 weeks and were then assigned to two groups matched by levels of responding: one group (dependent group) was exposed to chronic, intermittent ethanol vapors for 4 weeks to induce dependence; the other group (nondependent group) was not exposed to ethanol vapor. After a month of vapor exposure, rats were again tested during acute withdrawal (6-8 hours after removal from the vapor chambers) until stable levels of alcohol intake were achieved. As expected, alcohol vapor-exposed rats self-administered significantly greater amounts of alcohol than control rats not exposed to alcohol vapor during acute withdrawal. Rats were sacrificed during protracted abstinence (3 weeks after the end of alcohol vapor exposure) along with age-matched alcohol naive rats. 96 gene expression profiles (GEP) were obtained from 8 brain regions believed to be relevant in alcoholM-bM-^@M-^Ys reinforcing properties using the Affymetrix RN230.2 platform. Specifically, the following brain regions were microdissected and analyzed from nondependent and dependent alcohol self-administering rats as well as age-matched alcohol naive rats: (a) medial prefrontal cortex (MPF), (b) shell and (c) core NAc sub-regions, (d) central nucleus (CeA) and (e) basolateral nucleus of the amygdala (BLA), (f) dorsolateral and (g) ventral bed nucleus of the stria terminalis (BNST), and (h) ventral tegmental area (VTA).
2014-11-13 | E-GEOD-60966 | biostudies-arrayexpress
Project description:Objective: Postnatal glucocorticoids (GCs) are widely used in the prevention of chronic lung disease in premature infants. However, their use is associated with neurodevelopmental delay and cerebral palsy. We hypothesized that postnatal dexamethasone or betamethasone in high-dose, but not low-dose, would induce hypomyelination, astrogliosis, and motor impairment in premature rabbit pups. Additionally, these effects would be mediated by glucocorticoid receptors (GRs). Methods: Preterm rabbit pups, delivered by C-section at E29 (term=32d), were treated with a high dose of dexamethasone or vehicle. Myelin basic protein (MBP), glial fibrillary basic protein (GFAP), oligodendrocyte proliferation and maturation, and alteration of transcriptomic profile were evaluated in these pups. Neurobehavioral assessments were performed at 14d. Results: High-dose dexamethasone treatment reduced MBP expression and induced motor-impairment compared with controls. High-dose dexamethasone induced astrogliosis and altered genes associated with myelination, cell-cycle, GR, and MAP-Kinase signaling. Interpretation: High-dose postnatal dexamethasone arrested maturation of oligodendrocytes, and induced hypomyelination, gliosis and motor deficits. GC treatment reduced myelination by genomic GR-dependent mechanisms, and caused astrogliosis by non-genomic mechanisms. Two-condition experiment: forebrains of HDD (high dose dexamethasone) vs. CTR (PBS) post-natally expossed rabbit pups. Biological replicates: 4 CTR replicates, 4 HDD replicates.
2013-02-27 | E-GEOD-44610 | biostudies-arrayexpress
Project description:Rats were trained to orally self-administer alcohol in a concurrent, two-lever, free-choice contingency using a modification of the sweet solution fading procedure (O'Dell et al., 2004; Roberts et al., 2000; Vendruscolo et al., 2012). Following acquisition of self-administration, rats were allowed to self-administer unsweetened alcohol (10%) for 4 weeks and were then assigned to two groups matched by levels of responding: one group (dependent group) was exposed to chronic, intermittent ethanol vapors for 4 weeks to induce dependence; the other group (nondependent group) was not exposed to ethanol vapor. After a month of vapor exposure, rats were again tested during acute withdrawal (6-8 hours after removal from the vapor chambers) until stable levels of alcohol intake were achieved. As expected, alcohol vapor-exposed rats self-administered significantly greater amounts of alcohol than control rats not exposed to alcohol vapor during acute withdrawal. Rats were sacrificed during protracted abstinence (3 weeks after the end of alcohol vapor exposure) along with age-matched alcohol naive rats.
2014-11-13 | GSE60966 | GEO
Project description:Which proteins in oligodendrocytes require VAMP2/3-mediated exocytosis for membrane trafficking? We isolated primary oligodendrocytes from transgenic mice expressing oligodendrocyte-targeted botulinum toxin and littermate controls and used surface biotinylation to determine which proteins were differentially trafficked to the cell surface.
2022-10-14 | PXD036174 | Pride
Project description:Dose dependent reprogramming of neutrophils by LPS
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Project description:Dose-Dependent Hepacivirus Infection Reveals Linkage between Infectious Dose and Immune Response
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Project description:Objective: Postnatal glucocorticoids (GCs) are widely used in the prevention of chronic lung disease in premature infants. However, their use is associated with neurodevelopmental delay and cerebral palsy. We hypothesized that postnatal dexamethasone or betamethasone in high-dose, but not low-dose, would induce hypomyelination, astrogliosis, and motor impairment in premature rabbit pups. Additionally, these effects would be mediated by glucocorticoid receptors (GRs). Methods: Preterm rabbit pups, delivered by C-section at E29 (term=32d), were treated with a high dose of dexamethasone or vehicle. Myelin basic protein (MBP), glial fibrillary basic protein (GFAP), oligodendrocyte proliferation and maturation, and alteration of transcriptomic profile were evaluated in these pups. Neurobehavioral assessments were performed at 14d. Results: High-dose dexamethasone treatment reduced MBP expression and induced motor-impairment compared with controls. High-dose dexamethasone induced astrogliosis and altered genes associated with myelination, cell-cycle, GR, and MAP-Kinase signaling. Interpretation: High-dose postnatal dexamethasone arrested maturation of oligodendrocytes, and induced hypomyelination, gliosis and motor deficits. GC treatment reduced myelination by genomic GR-dependent mechanisms, and caused astrogliosis by non-genomic mechanisms.
2013-02-27 | GSE44610 | GEO