Dose dependent reprogramming of neutrophils by LPS
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ABSTRACT:
PROVIDER: PRJNA958111 | ENA |
REPOSITORIES: ENA
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Project description:We have performed analyses of murine primary bone marrow derived neutrophils challenged with either ultra-low dose or high dose of LPS. Neutrophils can be differentially programmed to distinct states by varying dosages of LPS. Purified bone marrow neutrophils were treated with PBS, 100 pg/ml LPS or 100 ng/ml LPS overnight, and harvested for scRNAseq analysis to examine their profiles of gene expression.
2023-12-27 | GSE230241 | GEO
Project description:We have generated immune-enhancing neutrophils by culturing murine primary bone marrow derived neutrophils with either super-low dose of LPS. Immune-enhancing neutrophils preferentially express co-stimulatory molecules such as CD74, CD44 and CD86, and exhibit reduced expression of CD11b. Purified bone marrow neutrophils were treated with PBS or 100 pg/ml LPS overnight in the presence of GM-CSF, and harvested for scRNAseq analysis to examine their profiles of gene expression.
2024-05-17 | GSE230237 | GEO
Project description:Generation of immune-enhancing neutrophils by subclinical super-low dose LPS
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Project description:Mice intranasally exposed to a low dose of LPS (i.e., 100 ng) are prone to develop features of allergic asthma upon subsequent exposure to house dust mites (HDM) allergens, while mice exposed to vehicle or 100 µg LPS do not develop such features. In order to understand the mechanisms that promote allergic asthma, we sought to characterize the lung neutrophils, which are massively recruited after LPS exposure, by single cell RNA-Seq.
2019-07-25 | E-MTAB-6902 | biostudies-arrayexpress
Project description:Deterministic reprogramming of neutrophils in tumors
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Project description:SHY2-dose dependent transcriptome analysis
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Project description:Human endogenous retroviruses (HERVs/MaLRs) are distributed among our 24 chromosomes and their long terminal repeats (LTRs) constitute putative regulatory sequences. HERVs have received much attention for their implication in cancer, autoimmunity and placental development. Herein, we used a recently described high-density microarray allowing the exploration of the whole HERVs/MaLRs transcriptome including 353,994 HERVs/MaLRs loci and also 1559 genes related to immunity. We obtained a first view of the HERV transcriptome in peripheral blood mononuclear cells (PBMCs) by using a composite panel of unstimulated, low dose and high dose LPS-stimulated PBMCs from healthy volunteers to mimic inflammatory response or monocyte anergy. About 5.6% of the HERVs/MaLRs repertoire is transcribed in PBMCs. Roughly, one tenth [5.7%-13.1%] of LTRs present a constitutive promoter or polyA function and a quarter [19.5%-27.6%] of LTRs may shift from silent to active LTRs, LTRs being broadly subjected to operational determinism. We provide evidence that some HERVs/MaLRs and genes share similar control of regulation upon LPS stimulation conditions, e.g. presenting a low dose LPS-dependent “tolerizable” profile which can be reversed by INF-g stimulation. Similarly to tissue tropism observed in solid tumors, stimulus-dependent response confirm that the expression of HERVs is tightly regulated in PBMCs. Altogether, these observations allow to integrate 62 HERVs/MaLRs and 26 genes in 11 canonical pathways. We highlight HERVs close to OAS2/3 and IFI44/IFI44L genes. HERV incorporation at the crossroads of immune response pathways, paves the way for further functional studies and analyses of HERV transcriptome in altered immune responses in vivo such as in sepsis.
2018-06-25 | GSE108239 | GEO
Project description:Polymorphonuclear cells (neutrophils) play an important role in the systemic inflammatory response syndrome and the development of sepsis. These cells are essential for the defense against microorganisms, but may also cause tissue damage. Therefore, neutrophil numbers and activity are considered to be tightly regulated. Previous studies have investigated gene transcription during experimental endotoxemia in whole blood and peripheral blood mononuclear cells. However, the gene transcription response of the circulating pool of neutrophils to systemic inflammatory stimulation in vivo is currently unclear. We examined neutrophil gene transcription kinetics in healthy human subjects (n=4) administered a single dose of endotoxin (LPS, 2 ng/kg iv). In addition, freshly isolated neutrophils were stimulated ex vivo with LPS, TNFM-NM-1, G-CSF and GM-CSF to identify stimulus-specific gene transcription responses. Whole transcriptome microarray analysis of circulating neutrophils at 2, 4 and 6 hours after LPS infusion revealed activation of inflammatory networks which are involved in signaling of TNFM-NM-1 and IL-1M-NM-1 and IL-1M-NM-2. The transcriptome profile of inflammatory activated neutrophils in vivo reflects extended survival and regulation of inflammatory responses. We show that these changes in neutrophil transcriptome are most likely due to a combination of early activation of circulating neutrophils by TNFM-NM-1 and G-CSF and a mobilization of young neutrophils from the bone marrow. After LPS infusion blood was taken at t=0, t=2, t=4 and t=6 hours. Neutrophils were isolated and gene expression of these cells was assessed. T=2, t=4 and t=6 were related to t=0 as control condition
2012-06-04 | E-GEOD-35590 | biostudies-arrayexpress
Project description:Shigella induces epigenetic reprogramming of zebrafish neutrophils
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Project description:Neutrophil gene transcription following lipopolysaccharide exposure. Microarray analysis of lipopolysaccharide-treated human neutrophils. Neutrophils respond to infection by degranulation, release of reactive oxygen intermediates, and secretion of chemokines and cytokines; however, activation of neutrophil transcriptional machinery has been little appreciated. Recent findings suggest that gene expression may represent an additional neutrophil function after exposure to lipopolysaccharide (LPS). We performed microarray gene expression analysis of 4,608 mostly nonredundant genes on LPS-stimulated human neutrophils. Analysis of three donors indicated some variability but also a high degree of reproducibility in gene expression. Twenty-eight verifiable, distinct genes were induced by 4 h of LPS treatment, and 13 genes were repressed. Genes other than cytokines and chemokines are regulated; interestingly, genes involved in cell growth regulation and survival, transcriptional regulation, and interferon response are among those induced, whereas genes involved in cytoskeletal regulation are predominantly repressed. In addition, we identified monocyte chemoattractant protein-1 as a novel LPS-regulated chemokine in neutrophils. Included in these lists are five clones with no defined function. These data suggest molecular mechanisms by which neutrophils respond to infection and indicate that the transcriptional potential of neutrophils is greater than previously thought.
2010-06-05 | E-GEOD-105 | biostudies-arrayexpress