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Formate supplementation enhances anti-tumor CD8+ T cell fitness and efficacy of PD-1 blockade

ABSTRACT: Formate supplementation enhances anti-tumor CD8+ T cell fitness and efficacy of PD-1 blockade

PROVIDER: PRJNA1003017 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25558000_1.fastq.gz	Fastqsanger.gz
	SRR25558000_2.fastq.gz	Fastqsanger.gz
	SRR25558001_1.fastq.gz	Fastqsanger.gz
	SRR25558001_2.fastq.gz	Fastqsanger.gz
	SRR25558002_1.fastq.gz	Fastqsanger.gz

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Formate supplementation enhances anti-tumor CD8+ T cell fitness and efficacy of PD-1 blockade

Project description:Metabolic support of the response to immune checkpoint blockage is unknown. We used scRNA-seq to determine the response of CD8+ cells to metabolic supplementation with the one carbon donor formate in combination with anti-PD-1

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Improved growth of Clostridium thermocellum AG553 via medium supplementation with formate and strain evolution

Project description:Strain AG553 was successfully constructed to eliminate side product formation during fermentation increasing the ethanol yield. Though ethanol yields were higher, the growth rate of the organism was significantly impaired. In order to improve growth rate, the organism was evolved and formate was added to the growth media. Both strategies successfully improved growth rate, but the mechanisms for that improved growth rate were still not understood. In order to get a better understanding of the mechanisms for improved growth, a proteomics and transcriptomics study of three strains: wild type, the unevolved AG553, and a representative evolved strain AG601 was completed with and without the addition of formate in the growth media.

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Formate-induced phospho-signalling

Project description:The mitochondrial serine catabolism to formate induces a metabolic switch to a hypermetabolic state with high rates of glycolysis, purine synthesis and pyrimidine synthesis. While formate is a purine precursor it is not obvious link between formate and pyrimidine synthesis. Methods Here we combine phospho-proteome and metabolic profiling to determine how formate induces pyrimidine synthesis. We discover that formate induces CAD phosphorylation. Mechanistically formate induces mTORC1 activity as quantified by S6K1 phosphorylation, which is known to phosphorylate CAD and increase its enzymatic activity. Treatment with the allosteric mTORC1 inhibitor rapamycin abrogates CAD phosphorylation and pyrimidine synthesis induced by formate. We conclude that formate activates mTORC1 and induces pyrimidine synthesis via increasing CAD activity.

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TNF blockade uncouples toxicity and anti-tumor efficacy induced with an agonist CD40 antibody

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Steady-state samples of Methanococcus maripaludis MM901 grown on formate under hydrogen limited conditions

Project description:Hydrogenotrophic methanogenic Archaea are defined by a H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. Hydrogenotrophs respond to H2 starvation both phenotypically and at the level of gene expression; however, the responses during growth on formate have not been characterized. Here we report that during continuous culture of Methanococcus maripaludis under defined nutrient conditions, growth yields relative to methane production decreased markedly with either H2 excess or formate excess, suggesting that energy spilling occurs. Using microarray analysis, we show that the expression of genes encoding F420-dependent steps of methanogenesis, including one of two formate dehydrogenases, increased with H2 starvation, but with formate occurred at high levels regardless of limitation or excess. One gene, encoding H2-dependent methylene-tetrahydromethanopterin dehydrogenase, decreased in expression with either H2 limitation or formate limitation. Expression of genes for the second formate dehydrogenase, molybdenum-dependent formylmethanofuran dehydrogenase, and molybdenum transport increased specifically with formate limitation. Of the two formate dehydrogenases, only the first could support growth on formate in batch culture where formate was in excess.

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FOXO1 enhances CAR T cell stemness, metabolic fitness and efficacy [human_FOXO1_scRNAseq]

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FOXO1 enhances CAR T cell stemness, metabolic fitness and efficacy [human_FOXO1_TCF7_OE_RNAseq]

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Effect of formate on LN229 cells

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Effect of formate on LN229 cells

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