Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of human neural stem cells (hNSCs) at 35degC and 37degC treatment.

ABSTRACT: Gene expression profile at single cell level of human neural stem cells (hNSCs) at 35degC and 37degC treatment.

PROVIDER: PRJNA1010697 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25791213_1.fastq.gz	Fastqsanger.gz
	SRR25791213_2.fastq.gz	Fastqsanger.gz
	SRR25791214_1.fastq.gz	Fastqsanger.gz
	SRR25791214_2.fastq.gz	Fastqsanger.gz
	SRR25791215_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Gene expression profile at single cell level of human neural stem cells (hNSCs) at 35degC and 37degC treatment.

Project description:In order to elucidate why mild hypothermia of 35degC could promote neurogenesis of cultured hNSCs, we performed single-cell RNA sequencing (scRNA-seq) to reveal the molecular mechanism.

2024-02-20 | GSE241889 | GEO

hNSCs siRNA NOVA2, PQBP1, BRPF1 and DYRK1A

Project description:inactivation of NOVA2 gene in human neural stem cells (hNSCs). Two independent series

2019-10-12 | GSE138766 | GEO

Expression data from Luteolin-treated human Neural Stem Cells (hNSCs)

Project description:Gene expression profiling reveals a potential role of Luteolin in human neuronal stem cells (hNSCs) differentiation . hNSCs purchased from Gibco were treated with 1 μM verbenalin for 24 hours. Microarray gene expression profiling was conducted for biological replicates of hNSCs cultured in differentiation cell culture medium supplemented with Luteolin for 24 hours and untreated control cells cultured in differentiation cell cultured medium .

2021-10-31 | GSE148160 | GEO

Expression data from Luteolin-treated human Neural Stem Cells (hNSCs)

Project description:Expression data from Luteolin-treated human Neural Stem Cells (hNSCs)

| PRJNA623355 | ENA

Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism [hNSCs]

Project description:We analyzed androgen receptor (AR) target genes on human neural stem cells (hNSCs) by ChIP-sequencing of AR IP after either DMSO (solvent), Testosterone 100nM, DHT 10 or 100nM 4 hours treatment in order to find the role of AR during brain development.

2018-02-01 | GSE86454 | GEO

MRNAs bound to RBM3 of hNSCs under differentiation conditions at 35℃ and 37℃.

Project description:To isolate RNA molecules bound to RBM3, we performed CLIP-seq analysis using differentiated hNSCs cultured in neural differentiation medium at 35℃ and 37℃.

2024-02-20 | GSE241548 | GEO

Gene expression data from human neural stem cells (hNSCs) treated with 10 uM TCQA for 24 hours

Project description:TCQA promoted differentiation of hNSCs at least toward the neuronal lineage and suggests the possibility of using TCQA to promote neurogenesis. When differentiation is induced by growth factor withdrawal, decreased expression of stemness gene and increased expression of NSC fate-promoting genes can be observed. Therefore, we evaluated the effect of TCQA on global gene expression in hNSCs during differentiation at 24h to elucidate the neurogenesis-promoting effects of TCQA.

2023-07-03 | GSE235835 | GEO

Quantitative proteomic analysis reveals the effects of human neural stem cells on multiple brain regions

Project description:Parkinson’s disease (PD) is one of the most common neurodegenerative disease caused by diminution of neurons in the substantia nigra (SN) which projects dopaminergic (DA) axons to the striatum and other target areas. Recently, accumulating data demonstrated the prospects of cell replacement therapy by using neural stem cell (NSCs) transplantation. However the mechanisms underlying the potential efficacy are not fully understood. To gain new insights into the mechanisms of 6-OHDA induced lesion and potential efficacy of hNSCs transplantation, Intrastriatal 6-Hydroxydopamine (6-OHDA) injected parkinsonian mice were unilaterally engrafted with undifferentiated human NSCs to striatum (ST). High-throughput quantitative proteomic approach was utilized to characterize the proteome profiles of PD related brain regions in these mice including SN, ST, olfactory bulb (OB) and subventricular zone (SVZ). The abundance of more than 5000 proteins with high confidence in each region was determined in this study which represents the most extensive proteomic study of PD mouse models to date. In addition to the disruption of the DA system, the quantitative analysis demonstrated the profound disturbance of SVZ proteome after 6-OHDA insult, in which the abundance of more than 20% proteins was significantly changed. After hNSCs engraftment, the proteome of SVZ was restored and the astrocytes in ST was greatly activated in companion with the increase in neurotrophic factors. Furthermore, bioinformatics analysis demonstrated that changes in proteome was not caused by the proliferation of hNSCs or their progeny, but rather by the reaction of endogenous cells. Overall, this study reveals that hNSCs benefits parkinsonian animals by eliciting endogenous responses in multiple brain regions, and discovers the unexpected role of SVZ cells in PD progress and treatment, providing new therapeutic targets.

2017-03-29 | PXD004152 | Pride

rAAVr3.45-IL10 infected human neural stem cells(hNSCs) gene expression analysis

Project description:The safety and efficacy(IL10 expression level) of a recombinant adeno-associated virus derivative (rAAVr3.45) were examined to evaluate its potential as a gene carrier for preparing interleukin-10 (IL10)-secreting human neural stem cells (HFT13) that can treat ischemic injuries or neurodegenerative diseases.

2017-05-18 | GSE99032 | GEO

hNSCs siRNA BRPF1, DYRK1A et PQBP1

Project description:hNSCs siRNA BRPF1, DYRK1A et PQBP1

| PRJNA1040232 | ENA