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PROVIDER: PRJNA1011507 | ENA |
REPOSITORIES: ENA
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Project description:Experimental design: 2 genotypes: PI230970 (resistant USDA Plant Introduction (PI) line containing SBR Rpp2 resistance gene) & Embrapa-48 (susceptible Brazilian cultivar) 2 treatments: Soybean rust challenge & mock infection 3 replications 10 time points: 6, 12, 18, 24, 36, 48, 72, 96, 120, 168hai TOTAL: 120 Affymetrix GeneChip(R) Soybean Genome Arrays ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel. The equivalent experiment is GM2 at PLEXdb.] genotype: PI230970 - time: 006 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 006 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: Mock(3-replications)
2011-11-22 | E-GEOD-33410 | biostudies-arrayexpress
Project description:Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.
2017-01-31 | PXD003126 | Pride
Project description:Experimental design: 2 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) & Cultivar Williams that does not have a known SBR resistance gene 2 treatments: Soybean rust (Phakopsora pachyrhizi) isolate Hawaii 94-1 & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 72 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94-1 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Steve Whitham. The equivalent experiment is GM37 at PLEXdb.]
2012-10-10 | GSE29741 | GEO