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ABSTRACT:
PROVIDER: PRJNA1015003 | ENA |
REPOSITORIES: ENA
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Project description:In order to gain insight into how bacteria respond to manganese (II) and relate to oxidation, the shotgun proteomics approach was applied to a potential Mn (II) oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). The LG1 strain, which grew equally well in these two conditions, presented a set of proteins related to the cellular processes vital to its survival and proteins that ensured its good adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation, but its expression was not modulated by the presence of Mn (II).Through these findings, we reiterate the ability of LG1 to be applied successfully in the bioremediation of Mn (II) environments and the efficiency of the shotgun approach in obtaining the first total shotgun proteome of this specie.
2018-08-04 | PXD010681 | JPOST Repository
Project description:Draft genome sequences of manganese-oxidizing bacteria
| PRJEB40009 | ENA
Project description:Whole genome sequence of Manganese oxidizing bacteria Pseudomonas resinovorans MO-1
| PRJDB5445 | ENA
Project description:Escherichia coli possesses >65 small proteins of <50 amino acids, many of which are uncharacterized. We have identified a new small protein, MntS, involved in manganese homeostasis. Manganese is a critical micronutrient, serving as an enzyme cofactor and protecting against oxidative stress. Yet manganese is toxic in excess and little is known about its function in cells. Bacteria carefully control intracellular manganese levels using the transcription regulator MntR. Before this work, mntH, which encodes a manganese importer, was the only gene known to respond to manganese via MntR repression in E. coli K12. We demonstrated that mntS is another member of the MntR manganese regulon. We also identified yebN, which encodes a putative manganese efflux pump, as the first gene positively regulated by MntR in Enterobacteria. Since MntS is expressed when manganese levels are low, causes manganese sensitivity when overexpressed, and binds manganese, we propose that MntS may be a manganese chaperone. This study reveals new factors involved in manganese regulation and metabolism and expands our knowledge of how small proteins function.
2011-12-08 | GSE25318 | GEO