Project description:SINEUPs are SINE repeat element containig long non-coding RNAs (lncRNAs)which positively regulate target mRNA translation at post-transcription level. SINE RNA region is crucial for SINEUP function. To analyze the effect of sequence deletion on RNA secondary structure of SINEB2 RNA derived from mouse natural antisense lncRNA to Gadd45α gene, RNA structure of four deletion mutants were chemically probed inside cells. For this, different structure mutants and target EGFP mRNA plasmids were co-transfected in HEK293 cells and icSHAPE libraries were prepared.
Project description:Deletion mutants of S. cerevisiae 4743 were grown in glucose-limited continuous chemostats (D = 0.2h-1). The deletion mutants used were: Homozygous deletion mutants of HAP4, OXA1, BCS1, RIP1, MBA1 and MIG1. Heterozygous deletion mutants of HAP4, RIP1 and MIG1.
Project description:NFYC-AS1 is an overlapping antisense RNA transcribed head-to-head to NFYC sense gene, encoding for the subunit C of NF-Y transcription factor, which is known as master regulator of cell cycle and proliferation in normal and tumor cells. Here we performed NFYC-AS1 silencing in lung squamous carcinoma H520 cells by Gapmer antisense oligonucleotides and CRISPR/Cas9 TSS deletion. Afterwards, we performed differentially expressed analysis and gene set enrichement analysis to investigate on NFYC-AS1 function and mechanism of action.
Project description:This dataset consists of 700 responsive mutants (four or more significant mRNA expression changes as a result of the deletion) from a gene expression profile compendium of 1,484 deletion mutants that have a role or are implicated in mRNA transcription regulation, mRNA turnover, signaling or are located in the nucleus.
Project description:This dataset consists of 784 non-responsive mutants (three or less significant mRNA expression changes as a result of the deletion) from a gene expression profile compendium of 1,484 deletion mutants that have a role or are implicated in mRNA transcription regulation, mRNA turnover, signaling or are located in the nucleus.