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Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis by DamID

ABSTRACT: Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis by DamID

PROVIDER: PRJNA1019641 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26134064_1.fastq.gz	Fastqsanger.gz
	SRR26134064_2.fastq.gz	Fastqsanger.gz
	SRR26134065_1.fastq.gz	Fastqsanger.gz
	SRR26134065_2.fastq.gz	Fastqsanger.gz
	SRR26134066_1.fastq.gz	Fastqsanger.gz

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Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis by DamID

Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E and escargot(esg) driven by c587Gal4ts

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Genome-wide DNA-binding analysis for Stat92E in Drosophila testis by DamID

Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E driven by c587Gal4ts;hopTum-l

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Genome-wide DNA-binding analysis for Stat92E in Drosophila testis by DamID

Project description:Genome-wide DNA-binding analysis for Stat92E in Drosophila testis by DamID

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The identification of Stat92E target genes in Drosophila testis by ChIP-seq [Stat-ChIP]

Project description:To identify the target genes of Stat92E in adult Drosophila testis cyst cells, the testes of c587ts;UAS-Stat92E were dissected and cross-linked to conduct chromatin immunoprecipitation(ChIP) assay and ChIP-high throughput sequencing.

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Project description:Purpose: Genome-wide DNA-binding analysis for Sox100B in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on Sox100B driven by ESG-Gal4 Results: 5046 significant genomic binding sites, corresponding to 3182 genes were identified via iDamID following Sox100B overexpression in progenitor cells compared to WT. About a third of these genes were in the list of significantly altered genes following Sox100B overexpression and/or depletion.

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Profiling of Esg genomic binding in the Drosophila midgut with DamID

Project description:Tissue stem cells divide asymmetrically to self-renew and generate differentiated progeny to maintain tissue homeostasis. Escargot (Esg) is a transcriptional repressor that is expressed in multiple stem cell populations in Drosophila melanogaster. Reduced esg function in intestinal stem cells (ISCs) causes a loss of ISCs and a biased differentiation of the daughter enteroblasts (EBs) toward the enteroendocrine (EE) cell fate. Loss of esg leads to an upregulation of differentiation factors and reduced Notch activity within EBs, indicating that Esg is a pivotal regulator of homeostasis in the intestine, supporting self-renewal to maintain a healthy stem cell pool as well as differentiation of progenitor cells. To identify potential transcriptional targets that mediate these phenotypes, in vivo DamID was used to generate the data deposited herein. 3 biological relicates were performed for Esg (with one dye-swap).

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Profiling of Esg genomic binding in the Drosophila midgut with DamID

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