Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E driven by c587Gal4ts;hopTum-l
Project description:Purpose: Genome-wide DNA-binding analysis for Stat92E and escargot(esg) in Drosophila testis cyst cells by DNA adenine methyltransferase identification(DamID).Methods: DNA adenine methyltransferase identification (DamID) on Stat92E and escargot(esg) driven by c587Gal4ts
Project description:To identify the target genes of Stat92E in adult Drosophila testis cyst cells, the testes of c587ts;UAS-Stat92E were dissected and cross-linked to conduct chromatin immunoprecipitation(ChIP) assay and ChIP-high throughput sequencing.
Project description:To identify the target genes of Stat92E in adult Drosophila testis cyst cells, the testes of control (c587ts) and c587ts;UAS-upd were dissected and cross-linked to conduct chromatin immunoprecipitation(ChIP) assay and ChIP-high throughput sequencing.
Project description:Purpose: Genome-wide DNA-binding analysis for E(spl)m8 in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on E(spl)m8 driven by Dl-Gal4 Results:
Project description:Comr protein was found to be a major regulator of gene activity in drosophila spermatocytes. We obtained Comr binding profile to determine targets of Comr. Comr binding in drosophila male germ line cells was determined using DamID technique.
Project description:Purpose: Genome-wide DNA-binding analysis for Sox100B in intestinal stem cells in Drosophila midgut by DNA adenine methyltransferase identification(DamID) Methods: DNA adenine methyltransferase identification (DamID) on Sox100B driven by ESG-Gal4 Results: 5046 significant genomic binding sites, corresponding to 3182 genes were identified via iDamID following Sox100B overexpression in progenitor cells compared to WT. About a third of these genes were in the list of significantly altered genes following Sox100B overexpression and/or depletion.
Project description:Comr protein was found to be a major regulator of gene activity in drosophila spermatocytes. We obtained Comr binding profile to determine targets of Comr. Comr binding in drosophila male germ line cells was determined using DamID technique. Comparison of Dam-Comr binding to Dam-alone signal in duplicate for each sample type.