Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq for Young Old mouse liver hepatocytes with and without high fat diet

ABSTRACT: RNAseq for Young Old mouse liver hepatocytes with and without high fat diet

PROVIDER: PRJNA1020550 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26157633_1.fastq.gz	Fastqsanger.gz
	SRR26157633_2.fastq.gz	Fastqsanger.gz
	SRR26157634_1.fastq.gz	Fastqsanger.gz
	SRR26157634_2.fastq.gz	Fastqsanger.gz
	SRR26157635_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

RNAseq for Young Old mouse liver hepatocytes with and without high fat diet

Project description:Our Goal was to determine how hepatocytes of young and old mice differentially respond to 9-weeks of high fat diet vs normal chow.

2024-04-17 | GSE243906 | GEO

Transcriptome analysis of brown fat in young or old mice in cold exposure

Project description:Purpose: We investigated the transcriptomic change in brown fat of young and old mice (wild type) through high-throughput RNA-sequencing (RNA-Seq) analysis when the mice were exposed to cold room or room temperatur. Methods: We prepared 10 of young (3 months) mice and 9 of old (24 months) mice, and kept them in cold room (4°c) or room temperature (24°c) for 24 hours. Then, we sacrified mice and extracted RNA from brown fat tissue (BAT) for RNA-seq experiment. Results: BAT of Young mice showed increased carbohydrate metabolism and glycolytic flux during cold exposure Conclusions: The thermogenesis function of BAT is accelerated on cold exposure.

2020-07-31 | GSE135391 | GEO

Transcriptomic profile of replicating NAFLD hepatocytes (from High-Fat High-Sucrose Diet - HFHS and Choline-Deficient High-Fat Diet - CDHFD).

Project description:C57Bl6/J male mice were put on different diets at 5 weeks of age, with a standard diet (SD) or a High-Fat High-Sucrose Diet (HFHS) or a Choline-Deficient High-Fat Diet (CDHFD) during 6 months. Primary hepatocytes cultures from 3 different models were synchronized in the cell cycle. Transcriptomic analysis was perfomed at 48hours of culture when HFHS and CDHFD hepatocytes harbor replication stress.

2020-07-11 | GSE154194 | GEO

Myc is required for adaptive beta cell replication in young mice but is not sufficient in one year old mice fed with a high fat

Project description:Purpose: To test the role of Myc in adaptive expansion of beta cells Methods: Islets were isolated from 8 week old or one year old male C57BL6 mice fed a standard or high fat diet. RNAseq and DNA mehtylseq was performed. Results: RNAseq showed that Myc target genes are upregulted in young, but not old mice after a short term high fat diet. DNA methylation analysis shows hypomethylation of cell cycle genes that are Myc targets in islets from young mice fed with a HFD. Importantly, DNA hypomethylation of Myc response elements does not occur in islets from one-year-old mice fed short-term HFD Conclusions: We conclude that Myc is required for metabolic stress-mediated beta cell expansion in young mice, but with aging Myc upregulation in beta cells is not sufficient to induce beta cell replication by, at least partly, an epigenetically-mediated resistance to Myc action.

2020-09-29 | GSE131941 | GEO

Expression data from epidermal stem cells from young mice fed either with normal diet or high fat diet

Project description:High fat diet (HFD), if prolonged, leads to obesity thereby accelerating the development of many ageing-related pathologies including chronic inflammation, cardiovascular disease, diabetes, and a higher predisposition to develop cancer. We asked whether HFD could reprogram the circadian output of young stem cells in a manner similar to what we observed during physiological ageing. HFD has been recently shown to induce a rewiring of the liver circadian transcriptome and metabolome, although in an obesity-independent manner. We therefore fed young (8 week old) C57Bl6 mice with HFD or its control diet for 7-weeks, a time when mice had not yet become obese, and performed circadian transcriptome analysis.

2017-08-10 | GSE84510 | GEO

Transcriptome analysis of brown fat in young or old mice in cold exposure

Project description:Transcriptome analysis of brown fat in young or old mice in cold exposure

| PRJNA558731 | ENA

mRNA expression of young, mid-old, and old fibroblasts

Project description:mRNA expression of young, mid-old, and old fibroblasts

| PRJNA971965 | ENA

TMT isobaric profiling of young versus old haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.

2023-10-16 | MSV000093126 | MassIVE

scRNA-seq of mouse liver tissue derived from young and old mice

Project description:We want to investigate how cells in the specific zones in murine liver are affected by age-related changes of the microenvironment. To this end, we generated high-quality scRNA-seq dataset of hepatocytes using Smart-seq3express from 2 young (3-5 months) and 2 old (18-20 months) male mice. Livers were perfused and viable hepatocytes were FACS-sorted based on size. In addition, we recorded ploidy levels of hepatocytes. We retained 545 hepatocytes in total after initial filtering.

2023-11-01 | E-MTAB-12579 | biostudies-arrayexpress

mRNA expression of young, mid-old, and old fibroblasts

Project description:Examination of the difference in mRNA expression profile between young, mid-old, and old fibroblasts

2023-05-19 | GSE232427 | GEO