Project description:Proteomics, lipidomics, and metabolomics analysis of extracellular vesicles secreted by an environmental strain and a clinical strain of Acanthamoeba castellanii.
Project description:Proteomics, lipidomics, and metabolomics analysis of extracellular vesicles secreted by an environmental strain and a clinical strain of Acanthamoeba castellanii.
Project description:Legionella pneumophila is a Gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle. One of its primary environmental hosts in the free-living amoeba Acanthamoeba castellanii and its infection by L. pneumophila mimics that seen in human macrophages. Here we present analysis of strand specific sequencing of the transcriptional response of L. pneumophila in broth growth and in infection of A. castellanii. Examination of 2 infection and 3 broth growth time points
Project description:Legionella pneumophila is a Gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle. One of its primary environmental hosts in the free-living amoeba Acanthamoeba castellanii and its infection by L. pneumophila mimics that seen in human macrophages. Here we present analysis of strand specific sequencing of the transcriptional response of L. pneumophila in broth growth and in infection of A. castellanii.
Project description:A culture of Acanthamoeba castellanii cells was infected with mimivirus. At specified times after infection, infected cells aliquots were subjected to total RNA extraction. Cy3 labeled cDNA was hybridized on custom designed Agilent oligonucleotide tiling microarrays covering both strands of the mimivirus genome.
Project description:Mutation in flagellar transcriptional regulatory gene (flrA) result in increased V. cholerae growth and intracellular survival in the amoeba host Acanthamoeba castellanii. To identify molecular mechanisms behind the increased survival and growth of the flrA mutant transcriptomics assay was done.
