Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

ABSTRACT: Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

PROVIDER: PRJNA1033345 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26557505.fastq.gz	Fastqsanger.gz
	SRR26557506.fastq.gz	Fastqsanger.gz
	SRR26557507.fastq.gz	Fastqsanger.gz
	SRR26557508.fastq.gz	Fastqsanger.gz
	SRR26557509.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 86

Similar Datasets

Translationally inhibited mRNAs control cell movement as untranslated sequences

Project description:Translationally inhibited mRNAs control cell movement as untranslated sequences

| PRJNA1035314 | ENA

Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

Project description:Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

| PRJNA1035318 | ENA

Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

Project description:Translation of localized mRNAs serves to regulate specific sites of protein synthesis and its functional activity, whereas translationally inhibited and untranslated mRNAs are compartmentalized for degradation or storage, with no recognized functional role. Here, however, we unexpectedly discover a group of functional, untranslated mRNA sequences that localize to integrin focal adhesions (FAs), dynamic multiprotein assemblies that govern cell movement. We show that specific mRNA sequences associate with native or mature FA functional states via messenger ribonucleoprotein (mRNP) complexes with the RNA and FA binding protein G3BP1, functioning to regulate cell migration. Mechanistically, the dimerizing propensity of the mRNA in G3BP1 mRNPs directly regulates FA protein function. Self-dimerizing mRNA sequences form large branched G3BP1 mRNPs, reducing FA protein turnover and extending adhesion lifetimes, inhibiting cell migration. In contrast, low self-dimerization promotes small more spheric G3BP1 mRNPs, facilitating FA complex dissolution and enhancing cell migration behaviors. Our findings define a previously unknown role for cytoplasmic mRNAs, challenging the notion that mRNAs are solely coding molecules and shedding light on their regulatory functions.

2024-04-20 | GSE246499 | GEO

Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

2024-04-20 | GSE246955 | GEO

Translationally inhibited mRNAs control cell movement as untranslated sequences

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-20 | GSE246956 | GEO

DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions.

Project description:Translation efficiency can be affected by mRNA stability and secondary structures, including G-quadruplex structures (G4s). The highly conserved DEAH-box helicase DHX36/RHAU resolves G4s on DNA and RNA in vitro, however a systems-wide analysis of DHX36 targets and function is lacking. We map globally DHX36 binding to RNA in human cell lines and find it preferentially interacting with G-rich and G4-forming sequences on more than 4500 mRNAs. While DHX36 knockout (KO) results in a significant increase in target mRNA abundance, ribosome occupancy and protein output from these targets decrease, suggesting that they were rendered translationally incompetent. Considering that DHX36 targets, harboring G4s, preferentially localize in stress granules, and that DHX36 KO results in increased SG formation and protein kinase R (PKR/EIF2AK2) phosphorylation, we speculate that DHX36 is involved in resolution of rG4 induced cellular stress.

| S-EPMC6547686 | biostudies-literature

Translational profiling reveals that mRNAs encoding cuticle collagens are translationally repressed upon nsun-1 knockdown

Project description:By comparing mRNAs contained in the polysome fraction to total mRNAs in Caenorhabditis elegans exposed to either RNAi control or nsun-1 RNAi, we found that loss of NSUN-1 translationally represses genes associated with collagens, cuticle integrity and development. These changes explain phenotypes of nsun-1 depletion, which include impaired fecundity, gonad extrusion and reduced barrier function.

2020-12-09 | GSE143618 | GEO

Untranslated regions of mRNAs.

Project description:Gene expression is finely regulated at the post-transcriptional level. Features of the untranslated regions of mRNAs that control their translation, degradation and localization include stem-loop structures, upstream initiation codons and open reading frames, internal ribosome entry sites and various cis-acting elements that are bound by RNA-binding proteins.

| S-EPMC139023 | biostudies-literature

Conserved 3'-untranslated region sequences direct subcellular localization of chaperone protein mRNAs in neurons.

Project description:mRNA localization provides polarized cells with a locally renewable source of proteins. In neurons, mRNA translation can occur at millimeters to centimeters from the cell body, giving the dendritic and axonal processes a means to autonomously respond to their environment. Despite that hundreds of mRNAs have been detected in neuronal processes, there are no reliable means to predict mRNA localization elements. Here, we have asked what RNA elements are needed for localization of transcripts encoding endoplasmic reticulum chaperone proteins in neurons. The 3'-untranslated regions (UTRs) of calreticulin and Grp78/BiP mRNAs show no homology to one another, but each shows extensive regions of high sequence identity to their 3'UTRs in mammalian orthologs. These conserved regions are sufficient for subcellular localization of reporter mRNAs in neurons. The 3'UTR of calreticulin has two conserved regions, and either of these is sufficient for axonal and dendritic targeting. However, only nucleotides 1315-1412 show ligand responsiveness to neurotrophin 3 (NT3) and myelin-associated glycoprotein (MAG). This NT3- and MAG-dependent axonal mRNA transport requires activation of JNK, both for calreticulin mRNA and for other mRNAs whose axonal levels are commonly regulated by NT3 and MAG.

| S-EPMC2878564 | biostudies-literature

Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

Project description:Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

| PRJNA145877 | ENA