Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translationally inhibited mRNAs control cell movement as untranslated sequences

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE246956 | GEO | 2024/04/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

Project description:Translation of localized mRNAs serves to regulate specific sites of protein synthesis and its functional activity, whereas translationally inhibited and untranslated mRNAs are compartmentalized for degradation or storage, with no recognized functional role. Here, however, we unexpectedly discover a group of functional, untranslated mRNA sequences that localize to integrin focal adhesions (FAs), dynamic multiprotein assemblies that govern cell movement. We show that specific mRNA sequences associate with native or mature FA functional states via messenger ribonucleoprotein (mRNP) complexes with the RNA and FA binding protein G3BP1, functioning to regulate cell migration. Mechanistically, the dimerizing propensity of the mRNA in G3BP1 mRNPs directly regulates FA protein function. Self-dimerizing mRNA sequences form large branched G3BP1 mRNPs, reducing FA protein turnover and extending adhesion lifetimes, inhibiting cell migration. In contrast, low self-dimerization promotes small more spheric G3BP1 mRNPs, facilitating FA complex dissolution and enhancing cell migration behaviors. Our findings define a previously unknown role for cytoplasmic mRNAs, challenging the notion that mRNAs are solely coding molecules and shedding light on their regulatory functions.

2024-04-20 | GSE246955 | GEO

Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

2024-04-20 | GSE246499 | GEO

Translationally inhibited mRNAs control cell movement as untranslated sequences

Project description:Translationally inhibited mRNAs control cell movement as untranslated sequences

| PRJNA1035314 | ENA

Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

Project description:Translationally inhibited mRNAs control cell movement as untranslated sequences [Ribo-Seq]

| PRJNA1035318 | ENA

Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

Project description:Translationally inhibited mRNAs control cell movement as untranslated sequences [RNA-seq]

| PRJNA1033345 | ENA

Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-09-05 | GSE31393 | GEO

DHX36 prevents the accumulation of translationally inactive mRNAs with G4-structures in untranslated regions.

Project description:Translation efficiency can be affected by mRNA stability and secondary structures, including G-quadruplex structures (G4s). The highly conserved DEAH-box helicase DHX36/RHAU resolves G4s on DNA and RNA in vitro, however a systems-wide analysis of DHX36 targets and function is lacking. We map globally DHX36 binding to RNA in human cell lines and find it preferentially interacting with G-rich and G4-forming sequences on more than 4500 mRNAs. While DHX36 knockout (KO) results in a significant increase in target mRNA abundance, ribosome occupancy and protein output from these targets decrease, suggesting that they were rendered translationally incompetent. Considering that DHX36 targets, harboring G4s, preferentially localize in stress granules, and that DHX36 KO results in increased SG formation and protein kinase R (PKR/EIF2AK2) phosphorylation, we speculate that DHX36 is involved in resolution of rG4 induced cellular stress.

| S-EPMC6547686 | biostudies-literature

Expression Profiles of mRNAs and microRNAs in HepG2 cells treated with Cyclosporin A and solvent control

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-27 | E-GEOD-45802 | biostudies-arrayexpress

Widespread shortening of 3' untranslated regions and increased exon inclusion characterize the human macrophage response to infection

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-09-06 | E-GEOD-73765 | biostudies-arrayexpress

Splenic fibroblasts control marginal zone B-cell movement and function via two distinct Notch2-dependent regulatory programs

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-10-18 | GSE274992 | GEO