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In vivo CRISPR screening of ANKL-PDX targeting to iron-requirement molecules

ABSTRACT: in vivo CRISPR screening of ANKL-PDX targeting to iron-requirement molecules

PROVIDER: PRJNA1033580 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26590055.fastq.gz	Fastqsanger.gz
	SRR26590056.fastq.gz	Fastqsanger.gz
	SRR26590057.fastq.gz	Fastqsanger.gz
	SRR26590058.fastq.gz	Fastqsanger.gz
	SRR26590059.fastq.gz	Fastqsanger.gz

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in vivo CRISPR screening of ANKL-PDX targeting to iron-requirement molecules

Project description:ANKL cell proliferates predominantly in liver sinusoid depending on transferrin. However, little is known about molecular mechanism of iron-dependency of ANKL. To investigate, we performed in vivo CRISPR screening of liver resident ANKL cells targeting to genes encoding 482 iron-requirement molecules.

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Project description:Iron and copper are important environmental nutrients for plant growth. However, the molecular mechanisms of both iron and copper signaling that integrate the two pathways remain poorly understood. The Arabidopsis thaliana high affinity copper transporter COPT5, is a tonoplast localized permease involved in copper remobilization. Here, a global expression microarray analysis of the copt5 mutant points out the induction of iron deficiency responses, including NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 4 (NRAMP4), a tonoplast-localized iron transporter. The copper requirement in iron perception and uptake from the media becomes more evident in the double nramp3nramp4 mutant, unable to remobilize iron from vacuoles, that is highly sensitive to copper deficiency. Furthermore, COPT5 expression is altered under iron deficiency and the copt5 mutant is sensitive to iron deficiency and is unable to perceive iron in the media under copper deficiency. Noteworthy, iron deficiency post-transcriptionally restraints the copper-dependent superoxide dismutase protein levels and the subsequent activity. As a consequence of its increased iron deficiency responses, the copt5 mutant present lower levels of both copper- and iron-dependent superoxide dismutase activities. Moreover, the copt5 mutant mobilizes faster its iron storage pools and presents higher levels of iron in cotyledons and seeds. These results underline the importance of internal metal pools in the understanding of copper and iron deficiency responses and their crosstalk that are critical for governing proper plant development in response to combined metal scarcities in soils.

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A prospective randomised trial in order to determine the benefit of preoperative oral iron supplementation for patients undergoing elective colorectal cancer resection

Project description:Primary objectives: 1.To determine whether giving iron tablets to patients for 2 weeks before a colorectal cancer operation prevents or reduces anaemia requiring blood transfusion. Primary endpoint: Blood transfusion requirement Secondary endpoints: Change in haemoglobin produced by oral iron supplementsSide effect profile produced by oral iron supplements

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Sequential CRISPR screening uncovers an intrinsic requirement for HHEX in safeguarding human pancreatic differentiation

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