Homo sapiens
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-9723
PROVIDER: PRJNA103635 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gingival Epithelial Cell Transcriptional Responses to Commensal and Opportunistic Oral Microbial Species.
| PRJNA99353 | ENA
Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral pathogenic Porphyromonas gingivalis and Aggregatibacter (formerly actinobacillus) actinomycetemcomitans. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Experiment Overall Design: Gingival epithelial HIGK cells were sham infected (CTRL) and infected with either the oral pathogenic P. gingivalis (Pg) or A. actinomycetemcomitans (Aa). These samples were hybridized to Affymetrix microarrays. Understanding how host cells have adapted to pathogens, and how barrier cells respond to limit their impact, provides a mechanistic biological basis of microbial disease in the mixed bacterial-human ecosystem of the oral cavity.
2009-04-24 | E-GEOD-9723 | biostudies-arrayexpress
Project description:Gingival Epithelial Cell Responses to a Complex Microbiome Comprising Commensal and Opportunistic Oral Microbial Species
| PRJNA113779 | ENA
Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by mono- and complex co-culture with oral commensal S. gordonii and pathogenic P. gingivalis. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Experiment Overall Design: Gingival epithelial HIGK cells were sham infected (CTRL) and infected with either the oral commensal S. gordonii (Sg) or the pathogenic P. gingivalis (Pg) as well as co-cultured in mixed cultures of Sg and Pg (Sg+Pg). These samples were hybridized to Affymetrix microarrays. Understanding how host cells have adapted to commensals, and how barrier cells respond to limit their impact, provides a mechanistic biological basis of health in the mixed bacterial-human ecosystem of the oral cavity and provides insight on how the degree of complexity of a microbiome influences this balance.
2009-07-18 | E-GEOD-12121 | biostudies-arrayexpress
Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by mono- and complex co-culture with oral commensal S. gordonii and pathogenic P. gingivalis. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Keywords: infection state
2009-07-14 | GSE12121 | GEO
Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral pathogenic Porphyromonas gingivalis and Aggregatibacter (formerly actinobacillus) actinomycetemcomitans. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Keywords: infection state
2008-03-01 | GSE9723 | GEO
Project description:Understanding the impact of the microbiota on oral mucosal epithelial barriers, particularly within the gingiva, is essential for elucidating the mechanisms underlying oral health and disease. The gingiva exhibits intricate tissue architecture and undergoes dynamic microenvironmental shifts influenced by various factors. Notably, microbial colonization emerges as an early-life factor that plays a significant role in shaping the functional and immunological maturation of the gingival epithelium. This study utilizes spatial transcriptomic analysis to unveil distinct immunological characteristics between the buccal and palatal regions of the murine gingiva, impacting natural alveolar bone loss. Moreover, the analysis identifies a significant influence of the microbiota on the development and function of the junctional epithelium, which faces the oral microbial plaque, offering new insights into neutrophil recruitment to this strategic location. Additionally, the microbiota is found to impact fundamental functions of the gingival epithelium, including proliferation kinetics and barrier sealing. In conclusion, this study emphasizes the crucial role played by the microbiota in regulating gingival barriers and brings attention to its unique influence on specific epithelial regions within the gingiva.
2024-12-18 | GSE269576 | GEO
Project description:Understanding the impact of the microbiota on oral mucosal epithelial barriers, particularly within the gingiva, is essential for elucidating the mechanisms underlying oral health and disease. The gingiva exhibits intricate tissue architecture and undergoes dynamic microenvironmental shifts influenced by various factors. Notably, microbial colonization emerges as an early-life factor that plays a significant role in shaping the functional and immunological maturation of the gingival epithelium. This study utilizes spatial transcriptomic analysis to unveil distinct immunological characteristics between the buccal and palatal regions of the murine gingiva, impacting natural alveolar bone loss. Moreover, the analysis identifies a significant influence of the microbiota on the development and function of the junctional epithelium, which faces the oral microbial plaque, offering new insights into neutrophil recruitment to this strategic location. Additionally, the microbiota is found to impact fundamental functions of the gingival epithelium, including proliferation kinetics and barrier sealing. In conclusion, this study emphasizes the crucial role played by the microbiota in regulating gingival barriers and brings attention to its unique influence on specific epithelial regions within the gingiva.
2024-12-18 | GSE269575 | GEO
Project description:Understanding the impact of the microbiota on oral mucosal epithelial barriers, particularly within the gingiva, is essential for elucidating the mechanisms underlying oral health and disease. The gingiva exhibits intricate tissue architecture and undergoes dynamic microenvironmental shifts influenced by various factors. Notably, microbial colonization emerges as an early-life factor that plays a significant role in shaping the functional and immunological maturation of the gingival epithelium. This study utilizes spatial transcriptomic analysis to unveil distinct immunological characteristics between the buccal and palatal regions of the murine gingiva, impacting natural alveolar bone loss. Moreover, the analysis identifies a significant influence of the microbiota on the development and function of the junctional epithelium, which faces the oral microbial plaque, offering new insights into neutrophil recruitment to this strategic location. Additionally, the microbiota is found to impact fundamental functions of the gingival epithelium, including proliferation kinetics and barrier sealing. In conclusion, this study emphasizes the crucial role played by the microbiota in regulating gingival barriers and brings attention to its unique influence on specific epithelial regions within the gingiva.
2024-12-18 | GSE269171 | GEO
Project description:In this paper, we first report that EC smoking significantly increases the odds of gingival inflammation. Then, we seek to identify and explain the mechanism that underlies the relationship between EC smoking and gingival inflammation via the oral microbiome. We performed mediation analyses to assess if EC smoking affects the oral microbiome, which in turn affects gingival inflammation. For this, we collected saliva and subgingival samples from EC users and non-users and profiled their microbial compositions via 16S rRNA amplicon sequencing. We then performed α-diversity, β-diversity, and taxonomic differential analyses to survey the disparity in microbial composition between EC users and non-users. We found significant increases in α-diversity in EC users and disparities in β-diversity between EC users and non-users.
2022-12-09 | GSE201949 | GEO