Attenuated retinoic acid signaling is among the early responses in mouse uterus approaching embryo attachment
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PROVIDER: PRJNA1039851 | ENA |
REPOSITORIES: ENA
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Project description:The uterus is transiently receptive for embryo implantation. It remains to be understood why the uterus does not reject semi-allogeneic or allogeneic embryos for implantation. Uterine early response genes at the time approaching embryo attachment may provide insights. Uteri from C57BL/6 pseudo-pregnant (PP) mice and pregnant (P) mice approaching embryo attachment on day 3 post-coitum (D3) @22 h were analyzed for early response genes by microarray. SAM algorithm retained 21,858 unique probesets. Principal component analysis (PCA) indicated a clear separation between the PP and P groups. There were 105 upregulated and 5 downregulated protein-coding genes in the pregnant uterus (fc>1.5 & q-value<5%). Gene ontology (GO) analysis of these upregulated genes revealed that 84% of the GO terms were related to immune responses, with a dominant natural killer (NK) cell activation signature. Among the top 8 upregulated protein-coding genes, Cyp26a1 inactivates retinoic acid (RA) while Lrat promotes vitamin A storage, both of which attenuate RA bioavailability; Atp6v0d2 and Gjb2 play roles in ion transport and transmembrane transport; Gzmb, Gzmc, and Il2rb are involved in immune responses; and Tdo2 is important for kynurenine pathway. Most of these genes or their related pathways have been implicated in immune regulations. RA signaling has been implicated in tolerance and immunity and uterine NK cells have been implicated in immunotolerance at the maternal-fetal interface in the placenta. The mechanisms of immune responses approaching embryo attachment remain to be elucidated. The coordinated effects of the early response genes may hold the keys to the question of why the uterus does not reject an implanting embryo.
2023-11-15 | GSE247638 | GEO
Project description:Human embryonic development after attachment remains elusive due to technical, ethical, and policy challenges of experimenting with early embryonic tissues. We build a model of the post-attachment human embryo by controllably assembling the stem cell model of the human epiblast with stem cell derived extraembryonic tissues. We provide three sets of single cell RNA sequencing data to characterize the system: data from the E10 in vitro attached IVF human embryo, stem cell-derived extraembryonic cells derived by BMP4 induction on soft substrates, and the resulting model of the post-attachment human embryo.
2022-06-02 | GSE202542 | GEO
Project description:Embryo implantation in the rabbit is unique in that a typical fusion type of implantation is employed, in which trophoblast cells adhere and fuse to the apical surface of uterine epithelial cells. In the present study, we analyzed global gene expression changes in the rabbit uterus during embryo implantation by using RNA-seq.
2015-12-18 | GSE76115 | GEO
Project description:The aim of this study was to examine differential gene expression profile in mouse uterus on day 5 of pregnancy between implantation sites and inter-implantation sites. There are 104,520 tags sequenced and acquired, 51,306 for non-implantation site, and 53,214 for implantation site. Keywords: Implantation, Uterus, Differential gene expression, Implantation sites and inter-implantation sites were isolated from the mouse uteri on day 5 of pregnancy from at least 20 mice. Implantation sites were identified by tail-vein injection of 0.1 ml of 1% Chicago blue 5 min before sample collection. SAGE analysis was used to examine differential gene expression in mouse uterus during embryo implantation.
2005-07-13 | E-GEOD-2892 | biostudies-arrayexpress
Project description:The role of bone morphogenetic protein 2 (Bmp2)in regulating the transformation of the uterine stroma during embryo implantation in the mouse was investigated by the conditional ablation of Bmp2 in the uterus using the (PR-cre) mouse. Experiment Overall Design: Bmp2 gene ablation was confirmed by real-time PCR analysis in the PR-cre; Bmp2fl/fl (termed Bmp2d/d) uterus. While littermate controls average 0.9 litter of 6.2 ± 0.7 pups per month, Bmp2d/d females are completely infertile. Analysis of the infertility indicates that whereas embryo attachment is normal in the Bmp2d/d as in control mice, the uterine stroma is incapable of undergoing the decidual reaction to support further embryonic development. Recombinant human BMP2 can partially rescue the decidual response, suggesting that the observed phenotypes are not due to a developmental consequence of Bmp2 ablation. Microarray analysis demonstrates that ablation of Bmp2 leads to specific gene changes, including disruption of the Wnt signaling pathway, Progesterone receptor (PR) signaling, and the induction of prostaglandin synthase 2 (Ptgs2). Taken together, these data demonstrate that Bmp2 is a critical regulator of gene expression and function in the murine uterus.
2008-04-04 | E-GEOD-10913 | biostudies-arrayexpress
Project description:The progesterone receptor A (PGRA) isoform is the dominantly expressed isoform within the mouse uterus and is indispensable for uterine function and fertility in the murine reproductive tract. During early pregnancy, the PGRA isoform is spatiotemporally expressed in the uterine epithelium. At days 2-3 of pregnancy, PGRA is upregulated within the epithelium and then decreases before day 4, the day of embryo implantation. This decrease of PGRA has been recognized as a marker to initiate the time in which the uterus is receptive to the embryo, yet this has never been functionally tested. We hypothesized the decrease of PGRA at the window of receptivity is required for successful embryo implantation. In order to test this hypothesis, we generated a PGRA conditional expression allele initiated by Cre recombinase present in the uterine epithelial compartment. The PGRA expressing mice exhibited infertility with the inability to undergo embryo implantation or decidualization. RNA microarray and ChIP-Seq analyses were utilized to identify the differentially regulated pathways controlled by PGRA at the time of implantation.
2019-08-29 | GSE86303 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE13858: Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment GSE13859: Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice Refer to individual Series
2010-06-24 | E-GEOD-14031 | biostudies-arrayexpress
Project description:Retinoic acid induces human gastruloids with posterior embryo-like structures
| PRJNA859692 | ENA
Project description:Using conditional knockout technology, we demonstrate that steroid receptor coactivator-2 (SRC-2) is essential for development of the receptive uterus for embryo implantation. Through bulk RNA-seq analysis, we reveal that endometrial SRC-2 is required for not only the induction of the expression of established genes associated with uterine receptivity but also for the expression of genes involved in endometrial epithelial changes that enable attachment and invasion of the embryo into the underlying stroma. Together, these RNA-seq results underscore the importance of SRC-2 as a transcriptional coregulator in the endometrium during the periimplantation period.
2023-11-20 | GSE237740 | GEO
Project description:We report the expression of mRNA and lncRNA in mouse uterus between periods of embryo implantation and labor by high-throughput seq. By the seq analysis of mRNA and lncRNA expression, 1971 differentially expressed (DE) mRNAs and 1543 DE lncRNAs were found between E0.5 and E4.5 at the embryo implantation stage, while 1149 DE mRNAs and 410 DE lncRNAs were found between E15.5 and E18.5 at the labor stage. While 117 DE mRNAs and 19 DE lncRNAs were commonly expressed between the two stages. This study provides a basic gene expression of altered pregnancy status.
2022-02-01 | GSE195795 | GEO