Project description:We report the construction of 5 yeast meiotic cDNA libraries and perform proof-of-principle screens to show that these yeast cDNA libraries can be used to identify genes and gene isoforms that are important for competitive fitness. Samples 1-25 are from different stages of cDNA library construction and deep sequencing was used to characterize gene representation in each yeast cDNA library. Samples 26-175 are from proof-of-principle competitive fitness screens.
Project description:In this proof-of-principle approach we have successfully applied a recombinant heterotypic histone modification interacting domain (HiMID) against H3K9me3 and H3K36me2/3 by using AND logic. We discovered that this new bivalent state is associated with the bodies of weakly transcribed genes and enhancers. Further, we also uncovered that this dual state is enriched in zinc finger coding genes and most likely connected to the zinc finger-Trim28-SetDB1 pathway.
Project description:This proof-of-principle experiment was designed to demonstrate the feasibility of proximity labeling for RNAM-bM-^@M-^Sprotein interactions IPL-seq on 293T-Rex expressing MSA-SNRPN70 (sample) or NFH-SNRPN70 (control)
