Project description:Wild-type (C57Bl6) mice were dark reared from birth to P42, then underwent monocular enucleation at P42. They were then placed in a normal light-dark environment for 4 days, after which they were sacrificed (at P46). Visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall Design: Gene expression in pooled samples from 2 non-deprived (ipsilateral to monocular enucleation) and 2 deprived (contralateral to monocular enucleation) visual hemispheres was compared. Note: two additional cross-animal comparisons were made.
Project description:To study the effect of sensory experience on 3D genome structure of the mouse brain, we performed Dip-C on 1,692 single cells from the visual cortex of dark-reared and control mice at different ages throughout the first postnatal month.
Project description:Microarray expression profiling of manually sorted m-citirin-labeled layer 4 visual cortex star pyramid neurons from deprived and non-deprived hemispheres.
Project description:Microarray expression profiling of manually sorted m-citirin-labeled layer 4 visual cortex star pyramid neurons from deprived and non-deprived hemispheres. Monocular deprivation by TTX-injection (at P12-13 and again at P13-14), followed by manual sorting of m-Citrin-labeled Layer 4 Visual Cortex Star Pyramid neurons in deprived and non-deprived hemispheres. RNA was extracted using PicoPure RNA Isolation Kit, reverse transcribed, and amplified using a standard T7 IVT protocol (Affymetrix Small Sample Target Labeling Assay Version II).
