Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA104815 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Identification of Transcriptional Targets Dual Functions Transcription Factor/Phosphatase Eyes Absent
| PRJNA103351 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE3286: Drosophila Life Cycle GSE3287: Unfertilized eggs GSE3288: Eyes absent mutant adults Abstract: Molecular genetic studies of Drosophila melanogaster have led to profound advances in understanding the regulation of development. Here we report gene expression patterns for nearly one-third of all Drosophila genes during a complete time course of development. Mutations that eliminate eye or germline tissue were used to further analyze tissue-specific gene expression programs. These studies define major characteristics of the transcriptional programs that underlie the life cycle, compare development in males and females, and show that large-scale gene expression data collected from whole animals can be used to identify genes expressed in particular tissues and organs or genes involved in specific biological and biochemical processes. Refer to individual Series
2006-03-06 | E-GEOD-4347 | biostudies-arrayexpress
Project description:Chromosomal clustering of genes in absent, small, or homeotic discs 2 (ash2) mutant larvae
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Project description:F2 progeny of AKHR mutant and obese parents and controls, 10-11 days old adults
| PRJNA382160 | ENA
Project description:Absolute (molar) quantification determines proteins stoichiometry in complexes, networks and metabolic pathways. We employed MS Western workflow to determine molar abundances of proteins critical for morphogenesis and phototransduction (PT) in eyes of Drosophila melanogaster using a single chimeric 264 kDa protein standard that covers, in total, 197 peptides from 43 proteins. Each protein was independently quantified with 2 to 4 proteotypic peptides with the coefficient of variation of less than 15 %, better than 1000-fold dynamic range and sub-femtomole sensitivity. We determined molar abundances and stoichiometric ratios of the components of the PT machinery and the rhabdomere, and how they are changing when rhabdomere morphogenesis is perturbed by genetic manipulation of the evolutionary conserved gene crumbs (crb).
2020-07-19 | PXD018001 | Pride
Project description:In Drosophila melanogaster larval hemolymph, under normal conditions, plasmatocytes and crystal cells represent respectively ~95% and ~5% of hemocytes, while lamellocytes, the third larval cell type, are absent since they are only induced after parasitoid wasp oviposition, their role being the encapsulation-melanization response to eliminate the wasp egg. However, even after induction lamellocytes number remains low, making difficult biochemical studies. Here using the D. melanogaster hopTum-l mutant that constitutively produces a high number of hemocytes, we set up a method to purify lamellocytes and analyzed their major proteins by 2D gel electrophoresis and their biotinylated plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry allowed to identify 430 proteins from the 2D spots and 344 from affinity purified proteins, totalizing 639 unique proteins. Known lamellocyte markers such as PPO3 and the integrin myospheroid are among the major proteins and affinity purification led to the detection of other integrins and a large array of integrins associated proteins involved in cell-cell junction formation and function. Overall newly identified proteins indicated that these cells are highly adapted to the encapsulation process but may have also several different physiological functions. This study provides the basis for new lamellocyte studies in vivo and in vitro, and develop markers to search whether different populations coexist, establish their origins and decipher their respective roles in drosophila physiology and immunity.
2024-04-29 | PXD016876 | Pride
Project description:Drosophila melanogaster technical error assessment using larvae and adults,
| PRJNA92287 | ENA
Project description:Genome-wide maps of KDM5 binding in drosophila adults
| PRJNA289118 | ENA
Project description:Eyes absent mutant adult flies collected 0-24 hours after eclosion, with N=2 biological collections for males and females each. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set
2005-09-10 | GSE3288 | GEO