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Mus musculus

ABSTRACT: RNA-seq of MC903-Induced atopic dermatitis-like mice using liquiritin

PROVIDER: PRJNA1050062 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27167920_1.fastq.gz	Fastqsanger.gz
	SRR27167920_2.fastq.gz	Fastqsanger.gz
	SRR27167921_1.fastq.gz	Fastqsanger.gz
	SRR27167921_2.fastq.gz	Fastqsanger.gz
	SRR27167922_1.fastq.gz	Fastqsanger.gz

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Project description:In order to analyze the regulatory effects of UII and GPR14 on gene transcription in Mc903-induced atopic dermatitis skin, ear skin collected from MC903 chronic itch mouse model on day 13 was used for in-depth analysis and sequencing of skin gene expression. The effect of GSK1562590 on the expression of pruritus and inflammatory factors in dermatitis was detected by RNA-seq.

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Project description:Atopic dermatitis was induced on dorsal skin of 7-10 week-old C57BL/6 female mice by bi- or tri-daily MC903 treatment. On day 8, viable mouse eosinophils (CD45+F4/80+SiglecF+) were sorted from skin regions of healthy or vehicle/treated atopic dermatitis or femur/tibia-derived bone marrow by FACS. Samples were paired within individual mice (4 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing.

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Effect of RGRN-305 on MC903-induced skin inflammation in mice

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Project description:The goal of the experiment is to assess the effect of GLPG2534 and dexamethasone in the MC903 mouse model for atopic dermatitis in a therapeutic setting.

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Single-cell transcriptomic profiling of dermal leukocytes

Project description:To decipher macrophage heterogeneity and kinetics during atopic dermatitis, dorsal skin of C57BL/6 female mice aged between 7-12 week-old received MC903 stimulation bi- or tri-daily. Dermal leukocyte-enriched single cells were prepared and analysed by single-cell RNA-seq in time course.

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RNA-sequencing of mouse Lang+ and Lang- migratory dendritic cells (DC) under 2 pathogenic conditions of atopic dermatitis

Project description:Comparison of mouse Lang+ and Lang- migratory dendritic cells (DC) under 2 pathogenic conditions of atopic dermatitis (MC903-treated and OVA treated)

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RNA-seq of tissues from MC903- and Ethanol-treated mice

Project description:We report transcript abundance in skin, spinal_cord, and trigeminal ganglia from animals treated with MC903 to induce a mouse model of atopic dermatitis across different genotypes, time points, and treatments

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The role of keratinocyte DDX5 in MC903-induced atopic dermatitis (AD) mice

Project description:To investigate whether DDX5 is involved in the development of atopic dermatitis, we performed gene expression profiling analysis using data obtained from RNA-seq of ear skins from Ddx5f/f and K14Ddx5f/f atopic dermatitis mice

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