Project description:The white button mushroom Agaricus bisporus is the most widely produced edible fungus with a great economical value. Its commercial cultivation process is often performed on wheat straw and animal manure based compost that mainly contains lignocellulosic material as a source of carbon and nutrients for the mushroom production. As a large portion of compost carbohydrates are left unused in the current mushroom cultivation process, the aim of this work was to study wild-type A. bisporus strains for their potential to convert the components that are poorly utilized by the commercial strain A15. Growth profiling suggested different abilities for several A. bisporus strains to use plant biomass derived polysaccharides, as well as to transport and metabolize the corresponding monomeric sugars. Six wild-type isolates with diverse growth profiles were compared for mushroom production to A15 strain in semi-commercial cultivation conditions. Transcriptome and proteome analyses of the three most interesting wild-type strains and A15 indicated that the unrelated A. bisporus strains degrade and convert plant biomass polymers in a highly similar manner. This was also supported by the chemical content of the compost during the mushroom production process. Our study therefore reveals a highly conserved physiology for unrelated strains of this species during growth in compost.
2018-05-22 | GSE99928 | GEO
Project description:Analysis of forest soil microbial community after treatment of spent mushroom substrate
| PRJNA1010876 | ENA
Project description:Succession of microbial community composition and network structure during composting of spent mushroom substrate
Project description:Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different precentage of olive mill solid waste (OMSW).
Mushroom fruit body (FB), spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.
Project description:Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different percentage of olive mill solid waste (OMSW). Mushroom fruit body (FB) spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS, using electro spray ionization (ESI) in a negative ion mode. (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.
