Project description:Examination of PPARg occupancy (GSE41481) and DNA hypersensitive sites (GSE122453) in in vitro differentiatied adipocytes isolated from epididymal and inguinal white adipose tissues, as well as brown adipose tissue.
Project description:Identifying gene expression changes in adipose tissue of lipodystrophic Pparg<ldi/+> targeted mice Experiment Overall Design: RNA from epididymal white adipose pads of three 10-week-old Pparg<ldi/+> males and three litter-matched WT controls was analyzed by MOE430v2.0 GeneChipâ?¢ arrays, one mouse per array.
Project description:We used microarrays to detail the gene expression profile during WAT -beige transition by treatment of beta adrenergic receptor agonist . Stromal vascular fractions (SVF) from mice (n = 3/group) that received vehicle or beta3 adrenergic receptor agonist, CL, treatment were served for RNA extraction and hybridization on Affymetrix microarrays. We are trying to find out angiogenic factors genes dynamics during white adipose tissues (WAT) - beige transition.