Project description:To test whether other genes were being silenced in the Cyp6g1 knockdown strain due to off-target RNAi effects, and whether other gene expression changes were contributing to the altered susceptibility to imidacloprid in these knockdown flies. A comparison between w;Act5C-GAL4/CyO; UAS:RNAi_Cyp6g1Hp2/TM3Sb and the genetic control w;Act-GAL4/CyO;+/TM3Sb was performed. Ten 2-3 day old adult males or females were transferred to sugar-agar plates and then collected at various time points (0, 2, 5, 8 hours). The RNA samples for up to three independent experiments per timepoint for each genotype were then pooled, in equal concentrations, before hybridisation to the Affymetrix Drosophila GeneChip 1. As a preliminary analysis provided evidence for assuming that there is no interaction between genotype and duration in the sugar-agar plate, the knockdown and control chips for each of the five timepoints provided some degree of replication for the comparison between the knockdown flies and control flies. Differential expression that was consistently observed between knockdown and control over each of the five timepoints was regarded as providing evidence of a genuine difference.
Project description:Chromosome conformation capture (4C-Seq) in Drosophila Twist-H2B embryos (carrying nuclear tag specifically in the mesoderm) during embryogenesis was performed, anchoring on 107 different viewpoints. Two timepoints (3-4hrs and 6-8hrs after egg laying) and two tissue context (whole embryo and mesoderm) were assayed. Two independent collections were performed at each timepoint.
Project description:The male terminalia of Drosophila melanogaster has undergone rapid morphological change between it and its closely related species, making it a useful model for studying evolution and development. In order to identify the network components that pattern the male terminalia, we isolated and sequenced whole RNA from two timepoints during pupal development, and next performed in situ hybridization for 100 highly expressed transcription factors during pupal development.
Project description:This SuperSeries is composed of the following subset Series:; GSE7644: CLKGR in S2 cells; GSE7646: CLK targets from fly heads; GSE7651: Timepoints 5073 strain; GSE7652: Timepoints Control strain; GSE7653: S2 cells transfected with Clk Experiment Overall Design: Refer to individual Series
Project description:We sequenced mRNA from 24 single D. melanogaster embryos (12 male and 12 female) taken from 8 early embryonic timepoints to generate the first sex specific timecourse of gene expression in early Drosophila development
