ABSTRACT: Discovery of novel members of the genera Carlavirus and Potexvirus, and detection of Nepovirus, Cilevirus, Betacarmovirus and Tobamovirus members in a single Hibiscus rosa-sinensis plant in Colombia using Metagenomic Sequencing
Project description:Background: Melatonin is considered to be a polyfunctional master regulator in animals and higher plants. Exogenous melatonin inhibits plant infection by multiple diseases; however, the role of melatonin in cucumber green mottle mosaic virus (CGMMV) infection remains unknown. Results: In this study, we demonstrated that exogenous melatonin treatment can effectively control CGMMV infection. The greatest control effect was achieved by 3 days of root irrigation at a melatonin concentration of 50 µM. Exogenous melatonin showed preventive and therapeutic effects against CGMMV infection at early stage in tobacco and cucumber. We utilized RNA sequencing technology to compare the expression profiles of mock-inoculated, CGMMV-infected, and melatonin+CGMMV-infected tobacco leaves. Defense-related gene CRISP1 was specifically upregulated in response to melatonin, but not to salicylic acid (SA). Silencing CRISP1 enhanced the preventive effects of melatonin on CGMMV infection, but had no effect on CGMMV infection. We also found exogenous melatonin has preventive effects against another Tobamovirus, pepper mild mottle virus (PMMoV) infection. Conclusions: Together, these results indicate that exogenous melatonin controls two Tobamovirus infection and inhibition of CRISP1 enhanced melatonin control effects against CGMMV infection, which may lead to the development of a novel melatonin treatment for Tobamovirus control.
2022-12-30 | GSE221904 | GEO
Project description:Chloroplast genome of Hibiscus rosa-sinensis (Malvaceae): Comparative analyses and identification of mutational hotspots
Project description:HIPP (heavy metal associated isoprenylated plant protein) is a group of metal-binding metallochaperones, which plays crucial roles in metal homeostasis and detoxification. Up to now, little information of CsHIPP family genes in C. sinensis has been reported. In present study, a total of 56 CsHIPP genes from C. sinensis were identified, which were unevenly distributed on 15 chromosomes. The CsHIPP family genes were divided into five groups based on the topology of phylogenetic tree and identified AtHIPPs and OsHIPPs. Motif and gene structure analysis showed that the members in the same group showed similar conserved motifs and intron/exon structure. The expression pattern of CsHIPP family genes in different organs of C. sinensis was investigated using the previous RNA-seq data. The expression level of CsHIPP genes in C. sinensis under Cd and As treatments was also explored based on our RNA-seq analysis. The results showed that 16 CsHIPP genes displayed the same expression pattern under Cd and As treatments, which were also verified by qRT-PCR. Among them, three CsHIPP genes (CsHIPP15, CsHIPP36, and CsHIPP4) were increased at both 10 d and 15 d after Cd and As treatments, and one gene (CsHIPP40) was increased to a maximum at 10 d, and decreased at 15 d of Cd and As treatments, indicating that these four CsHIPP genes in C. sinensis might be involved in the resistance to Cd and As stresses. These results provide a basis for the functional characterization of CsHIPP genes and investigations on the molecular mechanisms of heavy metal homeostasis in C. sinensis.
Project description:70mer probes were designed to detect plant viruses infection in genus level. This microarray platform is able to detect 169 plant virus species of 13 virus genera.
Project description:MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. Therefore, we employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissue-specific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5′ rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in Hibiscus syriacus. Collectively, this study provides the first reliable draft of the Hibiscus syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in Hibiscus syriacus.