Hermetia illucens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1064622 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Metatranscriptomics of the gut microbiome of black soldier fly larvae bred on lignocellulose-rich fiber diets
| PRJNA866094 | ENA
Project description:Transcriptome analysis of black soldier fly fat body and midgut.
| PRJDB14154 | ENA
Project description:Contamination of food products with mycotoxins such as aflatoxin B1 (AFB1) poses a severe risk to human health. Larvae of the black soldier fly (BSFL), Hermetia illucens (Diptera: Stratiomyidae), can successfully metabolize AFB1 without any negative consequences on their survival or growth. However, the underlying mechanisms that allow BSFL to metabolize AFB1 are unknown. In this study, five-day-old BSFL were fed with either a control or an AFB1-spiked (20 µg/kg) diet to elucidate the underlying mechanisms. Larval samples were collected at three timepoints (6 h, 24 h, and 72 h) and subjected to RNA-Seq analysis to determine gene expression patterns. Provision of an AFB1-spiked diet resulted in an up-regulation of 357 and a down-regulation of 929 unique genes. Upregulated genes include multiple genes involved in AFB1 metabolism in other (insect) species. Downregulated genes were generally involved in the insects' growth, development, and immunity. BSFL possesses a diverse genetic arsenal that encodes for enzymes capable of metabolizing AFB1 without trade-offs on larval survival.
2024-06-18 | GSE263746 | GEO
Project description:Nutritional immunology: Diversification and diet-dependent expression of antimicrobial peptides in the Black soldier fly Hermetia illucens
| PRJEB19091 | ENA
Project description:Black soldier fly larvae meal (BSFL) from Hermetia illucens is a promising alternative protein source in diets for farmed fish. The larvae can efficiently convert low-value organic material into high quality protein in a production cycle with low arable land and freshwater inputs. A few recent studies have shown that BSFL is a suitable protein source for Atlantic salmon (Salmo salar) in smaller controlled experiments. However, industry-relevant field trials conducted under large scale near-commercial conditions over a longer period are lacking. In this study, a feeding trial was performed to evaluate the impact of BSFL on growth performance and health of Atlantic salmon during the grow out phase in seawater, in a commercial site in Vestland county, Norway. A total of 320,000 post-smolt Atlantic salmon were distributed into six duplicate sea cages and fed one of three diets (commercial-like control diet and two test diets partially replacing the protein content of the control diet with 4 % and 8 % defatted BSFL meal) for 21 weeks, until a relevant commercial slaughter size of 4.5-5.0 kg was reached. Health parameters were assessed including histology of the distal intestine (DI), in addition to DI microbiota identification (by 16s rRNA-seq) and salmon RNA-seq of DI and head kidney (HK). The results showed that the inclusion of BSFL meal supported growth performance and had no adverse effect on gut health. The beta diversity of the distal intestine microbiota and the relative abundance of families Lactobacillaceae and the chitinolytic Bacillaceae increased in the fish fed the BSFL diets. Additionally, no histopathological changes were attributable to BSFL meal intake. Results from RNA-seq in DI revealed that BSFL inclusion modulates metabolic processes associated with lipids, the response to estrogens, the activity of immune receptors (to chemokines), phagocytosis and extracellular vesicles. Based on these results, black soldier fly larvae meal is a suitable alternative protein ingredient in inclusions of up to at least 8 % for Atlantic salmon under industrial fish farming conditions.
2024-08-16 | GSE241320 | GEO
Project description:The black soldier fly (Hermetia illucens) is important in antimicrobial peptides (AMP) research due to its exposure to diverse microbial environments. However, the impact of different fungal exposures on AMP abundance in H. illucens has not been thoroughly explored. Our study focused on basal conditions and interactions with three fungi: the non-pathogenic Candida tropicalis (isolated from larval gut), Saccharomyces cerevisiae, and the pathogenic Beauveria bassiana. Using RNA-seq and LC-MS/MS, we found that under standard conditions, the majority of AMPs belonged to the Lysozyme, Cecropin, and Defensin classes, with Defensins exhibiting the highest quantification levels. Exposure to any of the fungi upregulated AMP gene expression, indicating immune activation. Notably, exposure to C. tropicalis and B. bassiana led to notable downregulation of AMPs in H. illucens larvae compared to S. cerevisiae, suggesting these fungi may suppress or modulate the host immune response to aid their survival and colonization. The immune response of H. illucens larvae revealed that S. cerevisiae and B. bassiana trigger similar AMP pathways, whereas C. tropicalis elicits a distinct response with upregulation of Defensins and Cecropins. Lysozymes, known for their antibacterial and antifungal activity, were upregulated in response to S. cerevisiae and B. bassiana, but downregulated with C. tropicalis, potentially facilitating fungal survival in the larvae’s gut. This suggests that C. tropicalis adapts to reduce immune pressure, while B. bassiana may suppress AMPs to persist. Understanding these mechanisms opens possibilities for leveraging AMPs in combating C. tropicalis, which is implicated in human diseases.
2024-11-19 | GSE272202 | GEO
Project description:Decrypting the origin of bacterial and fungal communities on the black soldier fly egg surface
| PRJNA809118 | ENA
Project description:Farmed Atlantic salmon was given either a 6 % cellulose diet, a diet containing 6 % shrimp shell chitin or a diet containing 6 % chitin from black soldier fly larvae for a period of 4 weeks. The fish were split into six tanks at the beginning of the experiment; six fish per tank and two tanks per diet. RNA from stomach and pyloric caeca from four fish given each diet was sequenced.
2021-08-15 | E-MTAB-10594 | biostudies-arrayexpress
Project description:Phylogeography of the Black Soldier Fly Hermetia illucens based on mitochondrial COI sequences
| PRJEB36997 | ENA
Project description:Hermetia illucens breed:black soldier fly Genome sequencing and assembly
| PRJNA547968 | ENA