Project description:Transcriptional changes in field-grown plants of Vitis Vinifera cultivars 'Chardonnay' and 'Incrocio Manzoni' naturally infected with Bois Noir phytoplasma, compared to healthy samples. SUBMITTER_CITATION: Albertazzi G., Caffagni A., Milc J.A., Francia E., Roncaglia E., Ferrari F., Tagliafico E., Stefani E., Pecchioni N. (2009) Gene expression in grapevine cultivars in response to Bois Noir phytoplasma infection. Plant Science 176: 792-804. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Nicola Pecchioni. The equivalent experiment is VV14 at PLEXdb.] Experiment Overall Design: genotype: Chardonnay - disease type: Bois Noir infected(3-replications); genotype: Chardonnay - disease type: Healthy(3-replications); genotype: Incrocio Manzoni - disease type: Bois Noir infected(2-replications); genotype: Incrocio Manzoni - disease type: Healthy(2-replications)
Project description:Transcriptional profiling of Vitis vinifera cv. Chardonnay healthy vs. Phytoplasma-infected plants (Bois noir phytoplasma). Study was conducted on grapevine plants grown in the same vineyard (leaf midribs were sampled). Keywords: disease state analysis
Project description:Transcriptional changes in field-grown plants of Vitis Vinifera cultivars 'Chardonnay' and 'Incrocio Manzoni' naturally infected with Bois Noir phytoplasma, compared to healthy samples. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Nicola Pecchioni. The equivalent experiment is VV14 at PLEXdb.]
Project description:Transcriptional profiling of Vitis vinifera cv. Chardonnay healthy vs. Phytoplasma-infected plants (Bois noir phytoplasma). Study was conducted on grapevine plants grown in the same vineyard (leaf midribs were sampled). Keywords: disease state analysis Two-condition experiment: healthy vs. infected plants/shoots. Biological replicates: 4 healthy, 4 infected. No replicates. Each sample was prepared as a pool of several samples (each sample was collected from a different shoots/plants) of the same disease status. Each sample was co-hybridized to a common reference cRNA (pool of all samples).
Project description:Several systemic diseases affect Vitis vinifera worldwide with important consequent management costs. Phytoplasma and viruses represent the most detrimental pathogens inducing symptoms and metabolic alterations that modify quantitatively the crop production. In the aim to investigate the plant/pathogen interactions, different grapevine samples, naturally affected (in mixed or single infections) by Stolbur phytoplasma (agent of Bois Noir disease) and viruses, in comparison to healthy and recovered controls, to identify the plant response to systemic pathogen infection. The preliminary results showed that expression levels of thousands of genes were altered in infected plants, involving various metabolic pathways.
Project description:Several systemic diseases affect Vitis vinifera worldwide with important consequent management costs. Phytoplasma and viruses represent the most detrimental pathogens inducing symptoms and metabolic alterations that modify quantitatively the crop production. In the aim to investigate the plant/pathogen interactions, different grapevine samples, naturally affected (in mixed or single infections) by Stolbur phytoplasma (agent of Bois Noir disease) and viruses, in comparison to healthy and recovered controls, to identify the plant response to systemic pathogen infection. The preliminary results showed that expression levels of thousands of genes were altered in infected plants, involving various metabolic pathways. Total RNA was extracted from central leaf midribs and petioles from different V. vinifera cultivars in different conditions (healthy, infected and recovered). Microarray analyses were conducted using different biological replicates for treatment. The submitter of this dataset can no longer locate the raw data
Project description:The aim of the experiment was to identify important protein targets of phytoplasma infection, by isolation of plant proteins, interacting with phytoplasma effectors. Nicotiana benthamiana plants were transformed with YFP (Yellow Fluorescent Protein) tagged phytoplasma effector PoStoSP28, the leaves of transformed plants were homogenized and the effector was extracted together with its interactors through the YFP tag with a pull-down assay. As a control, the same procedure was performed with mock transformed plants and the samples analyzed with mass spectrometry.
