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RNA-Seq of Shiraz grapevines infected with GVA

ABSTRACT: RNA-Seq of Shiraz grapevines infected with GVA

PROVIDER: PRJNA1085026 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28369700_1.fastq.gz	Fastqsanger.gz
	SRR28369700_2.fastq.gz	Fastqsanger.gz
	SRR28369701_1.fastq.gz	Fastqsanger.gz
	SRR28369701_2.fastq.gz	Fastqsanger.gz
	SRR28369702_1.fastq.gz	Fastqsanger.gz

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Comparative proteomics of salt stressed own rooted and 110R grafted Thompson Seedless grapevines

Project description:Salt stress is a rising threat to agriculture system. The accumulation of salts near the plant roots hampers the normal uptake of water causing osmotic stress and ionic toxicity to the plant. Thompson Seedless is a popular table grape variety of Vitis vinifera L., which is sensitive to salt stress when grown on its own roots; grafting it onto a wild rootstock such as 110 Richtor (110R) makes it tolerant to salt stress. In the present study, shotgun-proteomics approach was used for the investigation of salt stress induced molecular response of own rooted and 110R grafted Thompson Seedless grapevines. A salt stress experiment was conducted on sixteen month old potted grapevines. The grapevines were treated with 150mM NaCl solution for seven days and the control vines were irrigated with tap water. The young leaf samples were collected from control and treated vines at three time-points viz. 6 hours, 48 hours and 7 days of salt stress. The stress responsive proteins identified through statistical analysis revealed a distinct response to salinity in both the vines.

2020-01-27 | PXD015515 | Pride

Vitis vinifera isolate:Clone 1654 | cultivar:Shiraz

Project description:Primary assembly of Vitis vinifera cv. Shiraz Clone 1654

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Expression data from 35S::VvCBF4-overexpressing grapevines

Project description:Overexpression of a grapevine C-repeat binding factor (CBF) gene, VvCBF4 in cv. Freedom was found to improve freezing survival in non-cold-acclimated vines. To understand the mechanistic basis of VvCBF4 transgene action, a transgenic line ('9-12') was genotyped using microarray-based mRNA expression profiling. Young grapevines (cv. 'Freedom') were grown in soil for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare vines transformed with the VvCBF4 transcription factor, expressed under the 35S CaMV promoter (line '9-12') vs. an empty vector transformant (line '8-6'). We harvested the whole aerial tissues of four biological replicates, each.

2011-07-08 | E-GEOD-29948 | biostudies-arrayexpress

RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines.

Project description:BackgroundGibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera 'Thompson Seedless' and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues.ResultsThe transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identified in the shoot, leaf and root tissues of the transgenic lines and these DEGs were involved in diverse biological processes; many of the DEGs showed strong tissue specificity and about 30% them carried a DELLA motif. We further discovered unexpected expression patterns of several key flowering induction genes VvCO, VvCOL1 and VvTFL1.ConclusionsOur results not only confirmed many previous DELLA study findings in annual species, but also revealed new DELLA targets and responses in grapevine, including the roles of homeodomain transcription factors as potential co-regulators with DELLA in controlling the development of grapevine which uniquely possess both vegetative and reproductive meristems at the same time. The contrasting responses of some key flowering induction pathway genes provides new insights into the divergence of GA-DELLA regulations between annual and perennial species in GA-DELLA signaling.

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Vitis vinifera isolate:Clone 1654 | cultivar:Shiraz

Project description:Genome sequencing and assembly of Vitis vinifera cv. Shiraz clone 1654

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Vitis vinifera isolate:Clone 1654 | cultivar:Shiraz

Project description:Haplotigs of the assembly of Vitis vinifera cv. Shiraz Clone 1654

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Scrapings from scale insect infested grapevines

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