Project description:Human primary aortic smooth muscle cells were infected with wild type (W50, 381), W50 derived gingipain mutants (E8 and K1A), or 381 derived fimbriae mutants (DPG3 and KRX178) strains of Porphyromonas gingivalis for 24 hours. The RNA was extracted from the cells and human whole genome microarray were used to measure gene expression.
Project description:Transcription profiling of P. gingivalis W50 grown in continuous culture under conditions of heme-excess and heme-limitation. Reference design (using Cy5 labelled genomic DNA as the reference) to compare two conditions: heme-excess vs heme-limitation. Three samples for each condition, independently grown.
Project description:Wild type Porphyromonas gingivalis strain ATCC33277 (V3176) and PG1626 - deficient mutant (V3177) were grown in iron replete conditions was used to compare to Porphyromonas gingivalis strains grown in iron chelated conditions.
