Project description:Transcription profiling of P. gingivalis W50 grown in continuous culture under conditions of heme-excess and heme-limitation. Reference design (using Cy5 labelled genomic DNA as the reference) to compare two conditions: heme-excess vs heme-limitation. Three samples for each condition, independently grown.
Project description:Human primary aortic smooth muscle cells were infected with wild type (W50, 381), W50 derived gingipain mutants (E8 and K1A), or 381 derived fimbriae mutants (DPG3 and KRX178) strains of Porphyromonas gingivalis for 24 hours. The RNA was extracted from the cells and human whole genome microarray were used to measure gene expression.
Project description:Reference design (using Cy5 labelled genomic DNA as the reference) to compare two genotypes: wild-type W50 vs an isogenic FeoB1 mutant W50FB1. Six biological replicates of both W50 and W50FB1, grown in continuous culture.