Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1087544 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We examined gene expression profiling of mussels treated with two chemical mixtures composed by heavy metals or organic compounds, both including genotoxic agents (cadmium, benzo[a]pyrene, 1-nitro/amino-pyrene) and other contaminants (cupper, mercury, fluoranthene, TCDD). For digestive gland one RNA sample from control mussels and one from each treatment group (7 mussels) were competitively hybridized on the MytArray 1.0. Total RNA was reverse-transcribed and labeled using a Cy3-dCTP and Cy5-dCTP direct incorporation. We carried out two separate hybridizations for each treatment conditions. Keywords = mussels Keywords = heavy metals Keywords = organic compaunds Keywords = gene expression profiling Keywords: ordered
2006-10-10 | GSE2183 | GEO
Project description:Colonization of deep-sea hydrothermal vents by invertebrates was made efficient through their adaptation to a symbiotic lifestyle with chemosynthetic bacteria, the primary producers of these ecosystems. Anatomical adaptations such as the establishment of specialized cells or organs have been evidenced in numerous deep-sea invertebrates. However, very few studies detailed global inter-dependencies between host and symbionts in these ecosystems. In this study, we proposed to describe, using a proteo-transcriptomic approach, the effects of symbionts on the deep-sea mussel Bathymodiolus azoricus’ molecular biology. We induced an in situ depletion of symbionts and compared the proteo-transcriptome of the gills of mussels in three conditions: symbiotic mussels (natural population), symbiont-depleted mussels and aposymbiotic mussels
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Project description:Fit phenotypes are achieved through optimal transcriptomic allocation. Here, we performed a high-resolution, multi-scale study of the transcriptomic tradeoff between two key fitness phenotypes, stress response (fear) and growth (greed), in Escherichia coli. We introduced 12 RNA polymerase (RNAP) mutations commonly acquired during adaptive laboratory evolution (ALE) and found that single mutations could result in large shifts in this fear vs. greed tradeoff likely through destabilizing the rpoB-rpoC interface. RpoS and GAD regulons drive the fear response while ribosomal proteins and the ppGpp regulon underlie greed. Growth rate selection pressure during ALE results in endpoint strains that universally have RNAP mutations, with synergistic mutations reflective of particular conditions. A phylogenetic analysis found the tradeoff in numerous bacteria species and one archaea species. The results suggest that the tradeoff represents a general principle of transcriptome allocation in bacteria where small genetic changes can result in large phenotypic adaptations to growth conditions.
2023-08-07 | GSE227624 | GEO
Project description:The present study tried to assess transcription level effects in the digestive gland of female mussels dietarily exposed to Ag NPs and to compare such transcription profiles in two different seasons, autumn and spring, since mussels are expected to be at a different gamete developmental stage (early and advanced gametogenic stage, respectively).
2019-01-01 | GSE124126 | GEO
Project description:The nacre color of shells has an effect on the pearl color in Hyriopsis cumingii, and is an important indicator for its value. However, little exosome and micro (mi)RNA information are available on nacre color formation in mussels. In this study, exosomes of mantles were extracted from white and purple mussels. High-throughput Illumina sequencing was performed on the white and purple mussel mantle exosomes.Moreover, miR-223 negatively regulated hcApo, which plays important roles in the absorption and transport of β-carotene in H. cumingii. These results improve our understanding of the molecular mechanisms of nacre color formation in H. cumingii.
2020-03-29 | GSE128650 | GEO
Project description:Transcriptional analysis of the effects of natural environmental variation across the vertical distribution of Mytilus californianus within a single mussel bed Keywords: Environmental Response 30 Biological replicates from plots sampled at 3 different verticle tide heights above the MLLW at Strawberry Hill Oregon. 15 mussels were sampled after a mid-day emmersion event and 15 mussels were sampled after a 1 hour recovery at ambient seawater temperatures. 1 replicate per array, compared using a common reference sample. 50 Biological replicates for 5 plots sampled at 2 different verticle tide heights above the MLLW at Boiler Bay Oregon. 25 mussels were sampled after a mid-day emmersion event and 25 mussels were sampled after a 1 hour recovery at ambient seawater temperatures. Pooled RNA from 5 biological replicates from each plot per array, compared using a common reference sample.
2011-12-25 | E-GEOD-21088 | biostudies-arrayexpress