Project description:The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.
Project description:Clavibacter michiganensis subsp. michiganensis is an important Gram-positive phytopathogenic bacteria that causes bacterial wilt and canker in tomato. The genome of the type strain, NCPPB382, has been sequenced and annotated, however comparative genomics suggests that certain regions are under- or misannotated. In order to improve the genome annotation, we have undertaken a proteogenomic study of this important pathogen. Samples were grown in culture and the proteome of the pellet and supernatant were analyzed separately using shotgun HPLC-MS/MS. These proteomics datasets were analyzed and a number of missing gene were found and a number of existing gene calls were modified.