Dataset Information

Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway

ABSTRACT: Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Rui Branca

LAB HEAD: Rui Branca

PROVIDER: PXD000005 | Pride | 2012-06-29

REPOSITORIES: Pride

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Publications

Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.

Arabi Azadeh A Ullah Karim K Branca Rui M M RM Johansson Johan J Bandarra Daniel D Haneklaus Moritz M Fu Jing J Ariës Ingrid I Nilsson Peter P Den Boer Monique L ML Pokrovskaja Katja K Grandér Dan D Xiao Gutian G Rocha Sonia S Lehtiö Janne J Sangfelt Olle O

Nature communications 20120101

Fbw7 is a ubiquitin-ligase that targets several oncoproteins for proteolysis, but the full range of Fbw7 substrates is not known. Here we show that by performing quantitative proteomics combined with degron motif searches, we effectively screened for a more complete set of Fbw7 targets. We identify 89 putative Fbw7 substrates, including several disease-associated proteins. The transcription factor NF-κB2 (p100/p52) is one of the candidate Fbw7 substrates. We show that Fbw7 interacts with p100 vi ...[more]

PMID: 22864569

Dataset Information

Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway

Publications

Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.

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