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Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (RNA-Seq)

ABSTRACT: Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (RNA-Seq)

PROVIDER: PRJNA1100501 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28687692.fastq.gz	Fastqsanger.gz
	SRR28687693.fastq.gz	Fastqsanger.gz
	SRR28687694.fastq.gz	Fastqsanger.gz
	SRR28687695.fastq.gz	Fastqsanger.gz
	SRR28687696.fastq.gz	Fastqsanger.gz

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Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (RNA-Seq)

Project description:Species of the genus Drosophila have served as favorite models in speciation studies, however genetic factors of the interspecific hybrid sterility are underinvestigated to date. Here we performed the analysis of reproductive incompatibilities of hybrid females in crossing Drosophila melanogaster females and Drosophila simulans males. Using transcriptomic data analysis, molecular, cellular and genetic approaches we analyzed differential gene expression, transposable element (TE) activity, piRNA biogenesis and functional defects of oogenesis in hybrids. A premature GSC loss was a most prominent defect of oogenesis in hybrid ovaries. Owing differential expression of genes encoding components of the piRNA pathway rhino and deadlock, functional RDCmel complex in hybrid ovaries was not assembled. At the same time the activity of RDCsim complex was maintained in hybrids, independently from the genomic origin of piRNA clusters. Despite identification of a cohort of overexpressed TEs in hybrid ovaries we found no evidences that their activity can be considered as the main cause of hybrid sterility. We revealed complex pattern of Vasa protein expression in hybrid germline, including partial AT-chX piRNA targeting of vasasim allele and significant developmental delay of vasamel expression. We came to the conclusions that complex multi-locus genetic changes between the species were responsible for hybrid sterility phenotype.

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Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans

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Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans (small RNA-Seq)

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Project description:see publication, arrays from 4 sterile genotypes containing homozygous segments with a Hybrid Male Sterility factor and 1 fertile strain in which the region is a Drosophila simulans and D. mauritiana heterozygous

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Molecular insights into female hybrid sterility in interspecific crosses between Drosophila melanogaster and Drosophila simulans

Project description:This SuperSeries is composed of the SubSeries listed below.

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Gene expression analysis of interspecific and intraspecific Y chromosome introgessions in Drosophila simulans

Project description:These arrays measure gene expression across eight Y introgression lines in Drosophila simulans. Four lines (Ya19, Ya23, Ya24, Ya26) carry a D. simulans Y chromosome (from a Cameroon population) and four lines (Sec01, Sec03, Sec08, Sec27) carry a D. sechellia Y chromosome. All lines are otherwise identical with a D. simulans background (UCSD stock center line 14021-0251.092). Four biological replicates of each of eight lines, plus two technical replicates (dye swap), for a total of 32 arrays. Full methods are described in the accompanying publication.

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Project description:Gene expression analysis of interspecific and intraspecific Y chromosome introgessions in Drosophila simulans

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