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Emergence of metabolic coupling to the heterotroph Alteromonas promotes dark survival in Prochlorococcus

ABSTRACT: Emergence of metabolic coupling to the heterotroph Alteromonas promotes dark survival in Prochlorococcus

PROVIDER: PRJNA1102011 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28738036.fastq.gz	Fastqsanger.gz
	SRR28738037.fastq.gz	Fastqsanger.gz
	SRR28738038.fastq.gz	Fastqsanger.gz
	SRR28738039.fastq.gz	Fastqsanger.gz
	SRR28738040.fastq.gz	Fastqsanger.gz

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Similar Datasets

Emergence of metabolic coupling to the heterotroph Alteromonas promotes dark survival in Prochlorococcus

Project description:Prochlorococcus is found throughout the euphotic zone in the oligotrophic open ocean. Deep mixing and sinking in aggregates or while attached to particles can, however, transport cells below this sunlit zone, depriving them of light for extended periods of time and influencing their circulation via ocean currents. Viability of these cells over extended periods of darkness could shape the ecology and evolution of the Prochlorococcus collective. We have shown that when co-cultured with a heterotrophic microbe and subjected to repeated periods of extended darkness, Prochlorococcus cells develop a heritable dark-tolerant phenotype – through an apparent epigenetic mechanism – such that they survive longer periods of darkness. Here we examine this adaptation at the level of physiology and metabolism in co-cultures of dark-tolerant and parent strains of Prochlorococcus, each grown with the heterotroph Alteromonas under diel light:dark conditions. The relative abundance of Alteromonas is higher in dark-tolerant than parental co-cultures, while dark tolerant Prochlorococcus cells are also larger, contain less chlorophyll, and are less synchronized to the light:dark cycle. Meta-transcriptome analysis of the cultures further suggests that dark-tolerant co-cultures undergo a coupled shift in which Prochlorococcus uses more organic carbon and less photosynthesis, and Alteromonas uses more organic acids and fewer sugars. Collectively, the data suggest that dark adaptation involves a loosening of the coupling between Prochlorococcus metabolism and the light:dark cycle and a strengthening of the coupling between the carbon metabolism of Prochlorococcus and Alteromonas.

2024-04-23 | GSE264347 | GEO

Impact of a heterotroph, Alteromonas MIT1002, on the transcriptome of Prochlorococcus NATL2A

Project description:We examine how the transcriptome of Prochlorococcus strain NATL2A changes in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002. Significant changes in the Prochlorococcus transcriptome were evident within six hours of co-culture, with groups of transcripts changing in different temporal waves. Many transcriptional changes persisted throughout the 48-hour experiment, indicating that the presence of the heterotroph affected a stable shift in Prochlorococcus physiology. These initial transcriptome changes largely correspond to reduced stress conditions within Prochlorococcus, as inferred from decreases in relative abundance for transcripts encoding DNA repair enzymes and many members of the âhigh-light inducibleâ family of stress response proteins. Notable changes were also seen in transcripts encoding components of the photosynthetic apparatus (particularly an increase in PSI subunits and chlorophyll synthesis enzymes), ribosomal proteins and biosynthetic enzymes. Changes in secretion-related proteins and transporters also highlight the potential for metabolic exchange between the two strains. At each of 7 timepoints, samples from 3 biological replicate co-cultures are compared to 3 biological replicate axenic Prochlorococcus cultures that serve as a control.

2016-06-10 | E-GEOD-73511 | biostudies-arrayexpress

Impact of a heterotroph, Alteromonas MIT1002, on the transcriptome of Prochlorococcus NATL2A

Project description:We examine how the transcriptome of Prochlorococcus strain NATL2A changes in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002. Significant changes in the Prochlorococcus transcriptome were evident within six hours of co-culture, with groups of transcripts changing in different temporal waves. Many transcriptional changes persisted throughout the 48-hour experiment, indicating that the presence of the heterotroph affected a stable shift in Prochlorococcus physiology. These initial transcriptome changes largely correspond to reduced stress conditions within Prochlorococcus, as inferred from decreases in relative abundance for transcripts encoding DNA repair enzymes and many members of the ‘high-light inducible’ family of stress response proteins. Notable changes were also seen in transcripts encoding components of the photosynthetic apparatus (particularly an increase in PSI subunits and chlorophyll synthesis enzymes), ribosomal proteins and biosynthetic enzymes. Changes in secretion-related proteins and transporters also highlight the potential for metabolic exchange between the two strains.

2016-06-10 | GSE73511 | GEO

What happens to Prochlorococcus when the sun does not come up?

Project description:We examine how the transcriptome of Prochlorococcus strain NATL2A changes in response to extended light deprivation, both when grown alone and in the presence of a naturally co-occurring heterotroph, Alteromonas macleodii MIT1002.

2018-05-01 | GSE93197 | GEO

Impact of a heterotroph, Alteromonas MIT1002, on the transcriptome of Prochlorococcus NATL2A

Project description:Impact of a heterotroph, Alteromonas MIT1002, on the transcriptome of Prochlorococcus NATL2A

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Project description:We report the full transcriptome of T4-like cyanophage P-HM2 infecting Prochlorococcus in light and dark

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