Project description:Our research goal is to illustrate the potential of gene expression profiling to discriminate between polluted and non-polluted field sites and predict the presence of a specific contaminant. Using a gene expression analysis, we challenged our custom Daphnia magna cDNA microarray to determine the presence of a specific metal toxicant in blinded field samples collected from two copper mines in California. We compared the gene expression profiles from our field samples to previously established expression profiles for Cu, Cd, and Zn. The expression profiles from the Cu containing field samples clustered with the Cu specific gene expression profiles. Many of the previously discovered copper biomarkers were also differentially expressed in the field samples, suggesting that gene expression analysis is capable of producing robust biomarkers of exposure, which can be validated in field studies. In addition, our study revealed that upstream field samples containing undetectable levels of Cu caused the differential expression of only a few genes, lending support for the concept of a No Observed Transcriptional Effect Level (NOTEL). If confirmed by further studies, the NOTEL may play an important role in discriminating polluted and non-polluted sites in future monitoring efforts. Keywords: ecotoxicogenomic exposure study

2008-06-09 | GSE7667 | GEO

Gene Expression changes induced by exposure to field water samples collected at Walker Mine, CA

2008-06-09 | GSE7666 | GEO

Oil field and Paddy Field soil samples

Project description:Comparative analysis of bacterial metagenome isolated from oil field and paddy field

| PRJNA876823 | ENA

16s Amplicon sequencing of soil samples from sorghum field

Project description:Amplicon sequencing of soil samples from sorghum field

| PRJEB15133 | ENA

Soil samples from field experiment with cover crops

Project description:Metagenome of soil samples from field experiment with cover crops

| PRJNA973547 | ENA