Project description:Diel proteomes of Trichodesmium colonies sampled from the field on March 10, 2018 from the Eastern subtropical Atlantic at 65 22.420W 17 0.284 N. These proteomes inform a broad study of diel proteome oscillations in Trichodesmium that support simultaneous photosynthesis and nitrogen fixation during the day.
Project description:Metaproteomes of individual Trichodesmium colonies collected from a single location in the Carribbean sea (65.22W, 17.02N) at 17:00 local time. Some colonies were associated with auto-fluorescent mineral particles. Their proteomes were analyzed individually to investigate the effect of the minerals on colony physiology.
Project description:This project presents field metaproteomics data from Trichodesmium colonies collected from the surface ocean. Most were collected from the tropical and subtropical Atlantic ocean, but there is also data from the long term Bermuda Atlantic Time Series and Hawaii Ocean Time Series. Trichodesmium is a globally important marine microbe and its growth and nitrogen fixation activity is limited by nutrient availability in the surface ocean. This dataset was generated to answer questions about limitations on Trichodesmium's growth and activity in the nature.
Project description:Twelve coral colonies of Montipora capitata were collected from patch reefs located in Kāne’ohe Bay, O’ahu, Hawai’i. Colonies were brought to shore where they were immediately split into two equally sized pieces, which were acclimated before bleaching. Half of each colony went to an increased water temperature treatment (30°C, bleaching treatment) and the other stayed at ambient temperature (25°C, nonbleached treatment) for three weeks. For the bleaching treatment, experimental tank temperatures were increased 2°C per day ( 1°C every 12 hours) for four days to a final temperature of 30°C. Samples were collected before bleaching (T1) and 24 hours after bleached colonies were returned to ambient temperature (T2), through 3 months post-bleaching (T6).
Project description:On August 22 – 25, 2017, 3 colonies of Montipora capitata with a diameter of ~ 24 cm were collected from the inner lagoon surrounding the Hawaii Institute of Marine Biology (HIMB). The corals were acclimated in outdoor flow-through tanks at HIMB that were supplied with ambient seawater and covered with shade-cloth to mimic PAR levels on the reef. Each colony was split in half with a hammer and chisel to produce two colonies of identical genotype, so that each genotype would experience both ambient and increased temperatures. On August 28 - 29, the corals were equally divided among the experimental tanks, at ambient (n=3) or increased (n=3) temperature. Throughout the temperature treatment, corals were randomly rotated among tanks to minimize potential tank effects. On September 1, the temperature in the increased temperature tanks was turned up 2°C per day, 1°C at 0900 and 1°C at 1400, for four days, reaching an average temperature of 30 °C. The ambient tanks averaged 25 °C. On September 26, the heaters were turned off and the temperature returned to ambient levels by Sept. 29.
Project description:Hybridization of one kidney of cortisol treated fish vs. one kidney of control fish. Kidneys were collected from untreated juvenile sea bream (n=4) and from fish, which received for 72h a coconut-oil implant containing 10mg/Kg (fish wet weight) (n=4) cortisol. Experiments were carried out at the University of the Algarve, Portugal in accordance with National legislation for the welfare of animals. Experiments were conducted in two 125 l cylindriconical tanks supplied with a continuous through-flow of oxygenated seawater at 20+1 °C using juvenile sea bream (25 g + 3 g) adapted for 1 week to the experimental conditions. One tank contained 8 untreated fish (control) and the other tank 8 cortisol treated fish and the end of experiments fish were removed form tanks, decapitated and the kidneys rapidly removed and place in RNAlater (Qiagen) at –20 °C. No mortality occurred in the control tank but 2 fish died in the cortisol treated tank. Keywords: other
2007-01-26 | GSE2064 | GEO
Project description:Combined Metagenomic and Metatranscriptomic analysis of individual Trichodesmium colonies
Project description:Hybridization of one kidney of cortisol treated fish vs. one kidney of control fish. Kidneys were collected from untreated juvenile sea bream (n=4) and from fish, which received for 72h a coconut-oil implant containing 10mg/Kg (fish wet weight) (n=4) cortisol. Experiments were carried out at the University of the Algarve, Portugal in accordance with National legislation for the welfare of animals. Experiments were conducted in two 125 l cylindriconical tanks supplied with a continuous through-flow of oxygenated seawater at 20+1 °C using juvenile sea bream (25 g + 3 g) adapted for 1 week to the experimental conditions. One tank contained 8 untreated fish (control) and the other tank 8 cortisol treated fish and the end of experiments fish were removed form tanks, decapitated and the kidneys rapidly removed and place in RNAlater (Qiagen) at â20 °C. No mortality occurred in the control tank but 2 fish died in the cortisol treated tank.