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Transcriptome profiles of wild-type, IRF8KO microglia

ABSTRACT: Transcriptome profiles of wild-type, IRF8KO microglia

PROVIDER: PRJNA1107154 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28873000_1.fastq.gz	Fastqsanger.gz
	SRR28873000_2.fastq.gz	Fastqsanger.gz
	SRR28873001_1.fastq.gz	Fastqsanger.gz
	SRR28873001_2.fastq.gz	Fastqsanger.gz
	SRR28873002_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiles of wild-type, IRF8KO microglia

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Single-cell transcriptome profiles of wild-type and IRF8KO microglia

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Project description:The role of the lineage-determining transcription factor IRF8 in microglia remains unelucidated. We analyzed the transcriptome diversity of wild-type and IRF8KO microglia. Deep-sequencing and subsequent differential analysis revealed the significance of the IRF8-dependent transcription on microglial heterogeneity and self-renewing. This study provides a novel insight into understanding IRF8-mediated transcriptional programs in microglia.

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Project description:The role of the lineage-determining transcription factor, Interferon regulatory factor (IRF8), in microglia remains elucidated. We report the genome-wide methyl-CpG status in adult wild-type (WT) and IRF8-knockout (IRF8KO) microglia by whole-genome bisulfite-Seq (WGBS). Deep-sequencing and subsequent differential analysis revealed that IRF8KO microglia exhibited a cell-intrinsic methylation profile. Furthermore, the differentially methylated regions showed a significant correlation with IRF8-dependent chromatin accessibility in microglia. This study provides a novel insight into understanding epigenetic regulation in microglia.

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