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ABSTRACT: Partial cyanobacterial phycocyanin gene sequences from Detroit Lake, Oregon, 2011-2012

PROVIDER: PRJNA1107260 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28877782.fastq.gz	Fastqsanger.gz
	SRR28877783.fastq.gz	Fastqsanger.gz
	SRR28877784.fastq.gz	Fastqsanger.gz
	SRR28877785.fastq.gz	Fastqsanger.gz
	SRR28877786.fastq.gz	Fastqsanger.gz

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Synergistic effect and mechanism study of Phycocyanin photosensitizer combined with Selenium against lung tumor in mouse

Project description:We used selenium as a photodynamic anti-tumor synergist of phycocyanin to explore its inhibitory effect on lung cancer and its molecular mechanism in vitro. First of all, we used LLC-luc mouse lung cancer cells to establish a tumor-bearing model. Selenium-enriched phycocyanin was injected next to the tumor. When it was absorbed by the tumor tissue, the tumor site was irradiated by a 620nm wavelength laser. The changes in tumor size were monitored in real-time and the physiological indexes of mice were measured. It was found that selenium phycocyanin photodynamic therapy could enhance the inhibitory effect of tumors and improve the level of antioxidation in tumor-bearing mice. In addition, the pathological section observation and electron microscope microstructure analysis of the tumor tissue showed that the effect of the selenium-enriched phycocyanin photodynamic treatment group was more significant. At the same time, the tumor tissue transcriptional group sequencing analysis and qRT-PCR verification analysis showed that selenium-enriched phycocyanin photodynamic treatment group could reduce the expression of Mmp13, Serpine1, Vegfa, and Ppbp genes inhibit tumor cell metastasis and proliferation, up-regulate the expression of Ccl2, Ccl3, Cxcl2 and down-regulate the expression of Ccl24 chemokine, and promote tumor local immunity. Our results show that selenium phycocyanin photodynamic therapy plays an anti-tumor effect by promoting tumor cell apoptosis, reducing inflammation, and promoting tumor immunity.

2023-01-01 | GSE192635 | GEO

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