Similar Datasets
Project description:We used selenium as a photodynamic anti-tumor synergist of phycocyanin to explore its inhibitory effect on lung cancer and its molecular mechanism in vitro. First of all, we used LLC-luc mouse lung cancer cells to establish a tumor-bearing model. Selenium-enriched phycocyanin was injected next to the tumor. When it was absorbed by the tumor tissue, the tumor site was irradiated by a 620nm wavelength laser. The changes in tumor size were monitored in real-time and the physiological indexes of mice were measured. It was found that selenium phycocyanin photodynamic therapy could enhance the inhibitory effect of tumors and improve the level of antioxidation in tumor-bearing mice. In addition, the pathological section observation and electron microscope microstructure analysis of the tumor tissue showed that the effect of the selenium-enriched phycocyanin photodynamic treatment group was more significant. At the same time, the tumor tissue transcriptional group sequencing analysis and qRT-PCR verification analysis showed that selenium-enriched phycocyanin photodynamic treatment group could reduce the expression of Mmp13, Serpine1, Vegfa, and Ppbp genes inhibit tumor cell metastasis and proliferation, up-regulate the expression of Ccl2, Ccl3, Cxcl2 and down-regulate the expression of Ccl24 chemokine, and promote tumor local immunity. Our results show that selenium phycocyanin photodynamic therapy plays an anti-tumor effect by promoting tumor cell apoptosis, reducing inflammation, and promoting tumor immunity.
2023-01-01 | GSE192635 | GEO
Project description:In this study, a co-expressed E. coli strain of phycocyanin-allophycocyanin was constructed, and co-expressed with chromophore in E. coli. Different molecular weights of phycocyanin and allophycocyanin were detected in the recombinant strains, indicating that the expressed polymers are different. The 66kDa dimer and the 300kDa multimer contained phycocyanin and allophycocyanin, identified by mass spectrometry.
2023-05-10 | PXD040154 | Pride
Project description:Chemotherapy-induced peripheral neuropathy (CIPN) is one of the most frequent side effects caused by antineoplastic agents, with a prevalence from 19% to over 85%. Clinically, CIPN is a mostly sensory neuropathy that may be accompanied by motor and autonomic changes of varying intensity and duration.
Due to its high prevalence among cancer patients, CIPN constitutes a major problem for both cancer patients and survivors as well as for their health care providers, especially because, at the moment, there is no single effective method of preventing CIPN; moreover, the possibilities of treating this syndrome are very limited.
The phycocyanin (PC), a biliprotein pigment and an important constituent of the blue-green alga Spirulina platensis, has been reported to possess significant antioxidant and radical-scavenging properties, offering protection against oxidative stress.
Study hypothesis is that phycocyanin may give protection against oxaliplatin-induced neuropathy in the treatment of gastro intestinal cancers including oesogastric, colo-rectal and pancreatic cancers. This trial will be a randomised placebo-controlled study.
| 2388220 | ecrin-mdr-crc
Project description:Synergistic effect and mechanism study of Phycocyanin photosensitizer combined with Selenium against lung tumor in mouse
| PRJNA792514 | ENA
Project description:Total protein and membrane-enriched fractions of Synechocystis sp. PCC 6803 wild type (WT) and mutant strains affected with inactivated DNA methyltransferase genes were analyzed by LC-HDMSE. The mutation of the DNA methyltransferase gene sll0729 encoding M.Ssp6803II initially led to a strong phenotype, including a lowered chlorophyll/phycocyanin ratio, impaired growth, and alterations in gene expression. Prolonged cultivation revealed instability of the initially obtained phenotype. Colonies showing normal pigmentation and WT-like growth appeared regularly and in high frequencies on agar plates. These colonies represent suppressor mutants, since the sll0729 gene is still completely inactivated and methylation of the M.Ssp6803II target GGCC sites does not occur. The proteomic analysis comprises the WT, two strains of suppressor mutants sll0729_1 and sll0729_15 as well as mutants of the DNA methyltransferase genes slr6095 and sll8009. For each of these five strains we investigated three biological replicates. Comparisons between the WT and these two suppressor mutant strains, but also between them and the slr6095 and sll8009 mutants enabled the detection of expression differences, which are specifically linked to the absence of M.Ssp6803II-related DNA methylation.
2019-06-26 | PXD012698 | Pride