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Differential transcriptomic profile of Piscirickettsia salmonis LF-89 and EM-90 during an in vivo spatial separation co-culture in Atlantic salmon

ABSTRACT: Differential transcriptomic profile of Piscirickettsia salmonis LF-89 and EM-90 during an in vivo spatial separation co-culture in Atlantic salmon

PROVIDER: PRJNA1108411 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28920200_1.fastq.gz	Fastqsanger.gz
	SRR28920200_2.fastq.gz	Fastqsanger.gz
	SRR28920201_1.fastq.gz	Fastqsanger.gz
	SRR28920201_2.fastq.gz	Fastqsanger.gz
	SRR28920202_1.fastq.gz	Fastqsanger.gz

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Cohabitation of Piscirickettsia salmonis genogroups (LF-89 and EM-90): Synergistic effect on growth dynamics

Project description:Piscirickettsia salmonis, the biological agent of SRS (Salmon Rickettsial Syndrome), is a facultative intracellular bacterium that can be divided into two genogroups (LF-89 and EM-90) with different virulence levels and patterns. There are studies that have found co-infection of these genogroups in salmonid farms in Chile, but it is essential to assess whether this competitive interaction within the host is related to virulence and changes in pathogen dynamics. In this work, we studied one isolate from each genogroup, EM-90 and LF-89 . The aim was to evaluate how co-cultures could affect their growth performance and virulence factors expression at in vivo cultures in Atlantic salmon. During in vivo co-cultures, transcriptomic analysis revealed an upregulation of transposases, flagellum-related genes (fliI and flgK), transporters and permeases that could unveil novel virulence effectors used in the early infection process of P. salmonis. Thus, our work has shown that the cohabitation of the genogroups of P. salmonis can modulate their behavior and virulence effectors expression. These data can contribute to new strategies and approaches to improve current health treatments against this salmonid bacterium.

2023-10-03 | GSE235725 | GEO

Differential transcriptomic profile of Piscirickettsia salmonis LF-89 and EM-90 during an in vivo spatial separation co-culture in Atlantic salmon

Project description:Salmonid Rickettsial Syndrome (SRS), caused by Piscirickettsia salmonis, is the most important disease in the Chilean aquaculture industry since it induces the highest mortality rates among infectious diseases. P. salmonis is a facultative intracellular bacterium comprising two genetically distinct groups (LF-89 and EM-90) in Chile. Previous data suggest that their cohabitation triggers the expression of virulence effectors, which could be related to a higher pathogenicity in salmon during a co-infection. Therefore, we evaluated whether the physical contact between two isolates from LF-89 and EM-90 was needed to activate this effect. Through a spatially separated in vivo co-culture inside Atlantic salmon followed by RNA-seq analysis, we compared the differential expressed genes (DEGs) with our previous results from an in vivo mixed co-culture. Data showed that LF-89 presented a similar virulence factor profile compared to the mixed co-culture. In contrast, EM-90 had more downregulated DEGs and the flagellar-related genes observed during mixed co-culture were absent. Hence, the synergistic effect related to increased pathogenicity to the host may be driven by the physical co-localization of both LF-89 and EM-90 P. salmonis isolates.

2025-01-22 | GSE266847 | GEO

Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89

Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89 Raw sequence reads

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Piscirickettsia salmonis EM-90

Project description:Piscirickettsia salmonis EM-90

| PRJNA231170 | ENA

Cohabitation of Piscirickettsia salmonis genogroups (LF-89 and EM-90): Synergistic effect on growth dynamics

Project description:Cohabitation of Piscirickettsia salmonis genogroups (LF-89 and EM-90): Synergistic effect on growth dynamics

| PRJNA987513 | ENA

Piscirickettsia salmonis LF-89 = ATCC VR-1361

Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Genome sequencing