Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1109557 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29009000.fastq.gz | Fastqsanger.gz | |||
| SRR29009001.fastq.gz | Fastqsanger.gz | |||
| SRR29009002.fastq.gz | Fastqsanger.gz | |||
| SRR29009003.fastq.gz | Fastqsanger.gz | |||
| SRR29009004.fastq.gz | Fastqsanger.gz |
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Project description:The goals of this study are to obtain the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis. Methods: O. furnacalis embryos were collected less than 12 h post oviposition. 1st, 3rd and 5th instar of the Asian corn borer, which represents the newly hatched larvae, the middle stage of larvae and the mature larvae, was harvested for subsequent experiments. Pupae and adults were grouped into females and males, then female and male samples were mixed at the ratio of 1:1. The 3rd instar larvae were anesthetized on an ice plate for tissue extraction. The midgut, fat body and silk gland were isolated. All these samples were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Results: We obtained the transcriptome profiling (RNA-seq) of different developmental stages and tissues in Ostrinia furnacalis Conclusions: Our study representsa detailed analysis of different developmental stages and tissues in Ostrinia furnacalis
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Project description:Our data on third instar larvae tissues of Drosophila melanogaster has allowed us to identify bsAS, a lncRNA antisense to the blistered/DSRF gene, orthologous to the SRF transcription factor in humans, mainly expressed in fly wings. bsAS controls the expression of different isoforms of the blistered gene in Drosophila tissues. CRISPR-CAS deletion of bsAS Transcription Start Site thoroughly abolishes bsAS transcription, inducing a change in the bs isoform usage and the impairment of wing development. Our data consists on stranded RNA-Seq from eye-antenna, leg and wing imaginal discs from third instar larvae. To analyse bsAS mutation, we also performed stranded RNA-Seq of wild type and bsAS-/- eyes and wings from third instar larvae and late pupae.
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