Project description:We report here the effect of vitamin K1 on gene expression in ARPE-19 cells with erastin induced iron death injury, in order to explore the important role and potential mechanism of vitamin K in iron death injury in RPE.
Project description:In this study, viral and cellular gene expression profiles were acquired after infecting ARPE-19 and MCF10A cells with strain TB40/E-BAC4 of human cytomegalovirus. ARPE-19 cells are permissive for HCMV infection, while MCF10A cells are semi-permissive. This data was acquired to study the effects of epithelial and mesenchymal cell states on HCMV infection. Despite being derived from a primary retinal epithelial explant, ARPE-19 cells require long periods of high confluency to display epithelial characteristics. Under the conditions used in this experiment, ARPE-19 cells are phenotyically mesenchymal, as shown in Golconda et al, Viruses, 2024. MCF10A cells display strong epithelial characteristics under the conditions used here.
Project description:We used microarrays to evaluate the effect of SRPIN803 on gene expression in ARPE-19 cells. ARPE-19 cells were treated with SRPIN803 (10 uM) or the negative control (0.1% DMSO) for 4 hours for Total RNA isolation and hybridization on Microarray.
Project description:To characterize the potential molecular pathway(s) affected by iron treatment and identify the one(s) responsible for C3 induction, we performed a whole genome microarray on untreated ARPE-19 cells and cells treated with 250 ?M FAC for 48h/2d. Gene expression was compared between untreated and FAC-treated ARPE-19 cells, with three biological replicates in each.
Project description:To identify the dysregulated lncRNA and mRNA expression in ARPE-19 cells underwent EMT, we established a TGF-β1 induced EMT model of ARPE-19 cells. ARPE-19 cells were treated with or without 10 ng/ml TGF-β1 for 48 h. Total RNA are extracted and subjected to microarray assay (Arraystar Human LncRNA Microarray V3.0)
Project description:We use RNA Sequencing to analyze the potential molecular mechanisms in human pancreatic cancer cell line BxPC3 cells after erastin and vitamin C treatment for 48h.
Project description:RAD21 ChIA-PET in human ARPE-19 cells For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:To investigate the overall gene expression pattern and explore potential drug targets of diabetic retinopathy, we used high glucose media to treat the main endothelial cells in retina, ARPE-19 cells, and then performed the gene expression profiling analysis containing mRNA and LncRNA information.