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Untreated and iron-treated ARPE-19 cell gene expression

ABSTRACT: To characterize the potential molecular pathway(s) affected by iron treatment and identify the one(s) responsible for C3 induction, we performed a whole genome microarray on untreated ARPE-19 cells and cells treated with 250 ?M FAC for 48h/2d. Gene expression was compared between untreated and FAC-treated ARPE-19 cells, with three biological replicates in each.

ORGANISM(S): Homo sapiens

SUBMITTER: Joshua Dunaief

PROVIDER: E-GEOD-67603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium.

Li Yafeng Y Song Delu D Song Ying Y Zhao Liangliang L Wolkow Natalie N Tobias John W JW Song Wenchao W Dunaief Joshua L JL

The Journal of biological chemistry 20150323 19

Dysregulation of iron homeostasis may be a pathogenic factor in age-related macular degeneration (AMD). Meanwhile, the formation of complement-containing deposits under the retinal pigment epithelial (RPE) cell layer is a pathognomonic feature of AMD. In this study, we investigated the molecular mechanisms by which complement component 3 (C3), a central protein in the complement cascade, is up-regulated by iron in RPE cells. Modulation of TGF-β signaling, involving ERK1/2, SMAD3, and CCAAT/enhan ...[more]

PMID: 25802332

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