Project description:We sequenced mRNA from 2 muscle samples of the large yellow croaker (Larimichthys crocea) taken from normal feeding fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker muscle undergoing fasting.
Project description:We sequenced mRNA from 4 liver samples of the large yellow croaker (Larimichthys crocea) taken from thermal stress treatment fish, normal temperature treatment fish, cold stress treatment fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker liver undergoing thermal stress, cold stress and fasting.
Project description:We sequenced mRNA from 2 muscle samples of the large yellow croaker (Larimichthys crocea) taken from normal feeding fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker muscle undergoing fasting. Muscle mRNA profiles of control group (M7C) and 21-day fasting group (M7E) were generated by RNA-seq using Illumina HiSeq 2500.
Project description:We sequenced mRNA from 4 liver samples of the large yellow croaker (Larimichthys crocea) taken from thermal stress treatment fish, normal temperature treatment fish, cold stress treatment fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker liver undergoing thermal stress, cold stress and fasting. Liver mRNA profiles of control group (LB2A), thermal stress group (LC2A), cold stress group (LA2A) and 21-day fasting group (LF1A) were generated by RNA-seq, using Illumina HiSeq 2000.
Project description:In order to explore the changes in protein expression of large yellow croaker Larimichthys crocea under high temperature stress, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to perform proteome analysis on the liver between thermal-tolerant and thermal-sensitive groups of large yellow croaker.
Project description:<p>Gut microbiota can modulate nutrient metabolism, considerably affecting host growth and development. However, mechanisms by which gut microbiota regulate large yellow croaker (LYC; Larimichthys crocea) growth remain unclear. We assessed gut contents of fast-growing male (IWHM) and female (IWHF) LYCs and slow-growing male (IWLM) and female (IWLF) LYCs and subjected the data to metagenomic and metabolomic analyses. Gut microbiota composition significantly differed among LYCs with different growth rates: Vibrio abundance was considerably lower in IWHM LYCs (2.53%) than in IWLM LYCs (41.47%); Arthrobacter D abundance was the highest in IWHF LYCs (8.19%) but the lowest in IWLF LYCs (1.52%). Moreover, 124 and 483 differential metabolites were noted in IWHM-IWLM and IWHF-IWLF LYC pairs, respectively. Finally, we established a model for gut microbiota-metabolite relationship in LYCs to understand molecular mechanisms by which gut microbiota regulates nutrient metabolism. Our results highlight differences in gut microbiota between LYCs with high and low growth rates and provide comprehensive insights into molecular mechanisms underlying LYC growth regulation by gut microbiota.</p>
